HEK-Blue™ IL-12 Cells
|HEK-Blue™ IL-12 Cells||Unit size||Cat. code||Docs||Qty||Price|
Human & Mouse IL-12 Reporter Cells
3-7 x 10e6 cells
IL-12 Reporter Cells
HEK-Blue™ IL-12 cells are designed to detect bioactive human and mouse IL-12 by monitoring the activation of the STAT-4 pathway.
These cells were generated by stably introducing the human genes for the IL-12 receptor and the genes of the IL-12 signaling pathway into HEK293 cells. Furthermore, these cells express a STAT4-inducible SEAP reporter gene.
Binding of IL-12 to the IL-12R on the surface of HEK-Blue™ IL-12 cells triggers a signaling cascade leading to the activation STAT-4 with the subsequent production of SEAP.
Detection of SEAP in the supernatant of HEK-Blue™ IL-12 cells can be readily assessed using QUANTI-Blue™.
APPLICATIONS: HEK-Blue™ IL-12 cells can be used to validate the functionality of recombinant native or engineered human or mouse IL-12.
They can also be used to screen for molecules that inhibit IL-12 signaling such as antibodies targeting IL-12 or IL-12R and inhibitors of TyK2/JAK2.
Response of HEK-Blue™ IL-12 cells to increasing concentrations of human and murine recombinant IL-12.
After a 24h incubation, the levels of SEAP were determined using QUANTI-Blue™, a SEAP detection reagent, and by reading the optical density (OD) at 630 nm.
|Human IL-12||4.2 ng/ml||13|
|Murine IL-12||4.1 ng/ml||11|
The response ratio was calculated by dividing the OD at 630 nm for the treated cells by the OD at 630 nm for the untreated cells.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Specificity: human and mouse IL-12
Detection range: 1 - 100 ng/ml for human and mouse IL-12Back to the top
- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml HEK-Blue™ Selection (250x concentrate)
- 1 ml Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA & Canada)Back to the top
Interleukin 12 (IL-12), formerly known as cytotoxic lymphocyte maturation factor (CLMF) and natural killer cell stimulatory factor (NKSF), is primarily produced by dendritic cells and macrophages.
It promotes the development of Th1 responses and is a powerful inducer of interferon gamma (IFN-γ).
IL-12 exerts its biological effect following binding to the IL-12 receptor (IL-12R), which is a heterodimeric receptor composed of two subunits, IL-12R-β1 and IL-12R-β2.
Binding of IL-12 to its receptor triggers a signaling pathway involving TyK2 (tyrosine kinase 2), JAK2 (Janus kinase 2) and STAT4 (signal transducer and activator of transcription 4) which results in the production of IFN-γ.
In HEK-Blue™ IL-12 cells, activation of the STAT4 pathway with IL-12 leads to the production of SEAP.Back to the top