HEK-Blue™ IL-17 Cells
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Human & Mouse IL-17 Reporter Cells
3-7 x 10e6 cells
IL-17 Reporter Cells
HEK-Blue™ IL-17 cells are designed to detect bioactive human and murine IL-17A, as well as human IL-17F and IL-17E (also known as IL-25), by monitoring the activation of the NF-κB and AP-1 pathways.
Interleukin 17 (IL-17) is a family of six cytokines; including the proinflammatory IL-17A, IL-17E and IL-17F, and the poorly understood IL-17B, IL-17C and IL-17D . IL-17A, IL-17E and IL-17F bind a heterodimeric surface receptor (IL-17RA and IL-17RC) triggering a signaling cascade that results in AP-1 and NF-κB activation.
HEK-Blue™ IL-17 cells were generated by stable transfection of HEK293 cells with:
- the human IL-17 receptor genes (IL-17RA and IL-17RC)
- the human Act1 adaptor gene
- an NF-κB/AP-1-inducible SEAP reporter gene
Of note, these cells respond specifically to IL-17 as TNF-α and MyD88-dependent IL-1β responses have been blocked.
Upon IL-17 stimulation, HEK-Blue™ IL-17 cells trigger the activation of NF-κB/AP-1 and the subsequent secretion of SEAP. The levels of NF-κB/AP-1-induced SEAP can be readily monitored using QUANTI-Blue™.
HEK-Blue™ IL-17 cells can be used to validate the functionality, toxicity, and variable dosage effects of human or murine IL-17.
1. Gu C. et al., 2013. IL-17 family: cytokines, receptors and signaling. Cytokine. 64(2):477-85.
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Specificity: human and mouse IL-17A, human IL-17F and IL-17E (IL-25)
- 0.3 - 100 ng/ml for human and mouse IL-17A
- 0.3 - 100 ng/ml for human IL-17F
- 1- 100 ng/ml for human IL-17E (IL-25)