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HEK-Blue™ IL-17 Cells

HEK-Blue™ IL-17 Cells Unit size Cat. code Docs Qty Price
Human & Mouse IL-17 Reporter Cells
3-7 x 10e6 cells
hkb-il17
+-
$1,182.00

You may also need : Recombinant human IL-17A | View more associated products

July 3rd 2019 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

HEK-Blue™ IL-17 Cells signaling pathway
HEK-Blue™ IL-17 Cells signaling pathway

 

IL-17 Reporter Cells

HEK-Blue™ IL-17 cells are designed to detect bioactive human and murine IL-17A, as well as human IL-17F and IL-17E (also known as IL-25), by monitoring the activation of the NF-κB and AP-1 pathways. These cells can be used for screening anti-IL-17 antibodies. 

Interleukin 17 (IL-17) is a family of six cytokines; including the proinflammatory IL-17A, IL-17E and IL-17F, and the less studied IL-17B, IL-17C and IL-17D [1]. Notably, IL-17A and IL-17F play an important role in Th17 immunity against extracellular pathogens [1.] These cytokines are also implicated in tumorigenesis and autoimmune diseases, such as psoriasis and arthritis [2]. Multiple efforts have focused on blocking these cytokines with antibodies [1-2]. IL-17A and IL-17F bind a heterodimeric surface receptor (IL-17RA and IL-17RC) triggering a signaling cascade that results in AP-1 and NF-κB activation. 

HEK-Blue™ IL-17 cells were generated by stable transfection of the human genes encoding the IL-17RA/IL-17RC heterodimeric receptor and Act1 adaptor molecule into the human embryonic kidney HEK293 cell line. HEK-Blue™ IL-17 cells also express an NF-κB- and AP-1-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene. Of note, these cells respond specifically to IL-17 as TNF-α and MyD88-dependent IL-1β responses have been blocked. For your convenience, InvivoGen provides QUANTI‑Blue™ Solution, an easy and rapid means to detect and quantify SEAP activity in the cell culture supernatant.  

 

Features of HEK-Blue™ IL-17 cells:

  • Fully functional IL-17 signaling pathway
  • Readily assessable SEAP reporter activity
  • Functionally tested
  • Guaranteed mycoplasma-free

Applications of HEK-Blue™ IL-17 cells:

  • Detection of both human and mouse IL-17A
  • Detection of human IL-17E and IL-17F
  • Screening of anti-IL-17 or anti-IL-17 receptor antibodies
     

Reference:

1. Gu C. et al., 2013. IL-17 family: cytokines, receptors and signaling. Cytokine. 64(2):477-85.
2. Pappu R. et al., 2011. The interleukin-17 cytokine family: critical players in host defence and inflammatory diseases. Immunology. 134: 8-16.
 

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Specifications

Antibiotic resistance: Blasticidin, Hygromycin, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Specificity: human and mouse IL-17A, human IL-17F and IL-17E (IL-25)

Detection range:

  • 0.3 - 100 ng/ml for human and mouse IL-17A
  • 0.3 - 100 ng/ml for human IL-17F
  • 1- 100 ng/ml for human IL-17E (IL-25)

 

These cells are covered by a Limited Use License (See Terms and Conditions).

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Contents

  • 1 vial containing of  3-7 x 106 cells
  • 2 x 1 ml HEK-Blue Selection (250X concentrate)
  • 1 ml Normocin™ (50 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)

Shipped on dry ice (Europe, USA & Canada)

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