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IL-11 Reporter HEK 293 Cells

HEK-Blue™ IL-11 Cells Unit size Cat. code Docs Qty Price
Human IL-11 Reporter Cells
3-7 x 10e6 cells
hkb-hil11r
+-
$1,304.00

You may also need : HEK-Blue™ Selection | View more associated products

HEK-Blue™ IL-11 Cells signaling pathway
HEK-Blue™ IL-11 Cells signaling pathway

Interleukin-11 Reporter Cells

HEK-Blue™ IL-11 cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive interleukin-11 (IL-11) by monitoring the activation of the JAK/STAT3 pathway.  IL-11 has pleiotropic physiological functions, ranging from development and tissue homeostasis, to immune responses [1, 2].

 More details

 

Cell line description:

HEK-Blue™ IL-11 cells were generated by stable overexpression of the genes encoding the alpha chain of human IL-11 receptor (IL-11Rα), human STAT3, and a STAT3-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. STAT3-dependent SEAP activity is readily assessable in the supernatant using QUANTI-Blue™ Solution, a detection reagent. 
Of note, HEK-Blue™ IL-11 cells also respond to human IL-6 and IL-27 (see Figures) as they endogenously express the gp130 co-receptor shared by IL-11 and other cytokines of the IL-6 family.

Features of HEK-Blue™ IL-11 cells:

  • Fully functional IL-11 signaling pathway
  • Readily assessable STAT3-inducible SEAP reporter activity
  • The stability for 20 passages has been verified
  • Functionally tested and guaranteed mycoplasma-free

Applications of HEK-Blue™ IL-11 cells:

  • Detection of human IL-11 (0.1 - 100 ng/ml) and  murine IL-11 (0.1 -100 ng/ml)
  • Screening of anti-IL-11 and anti-IL-11Rα antibodies

 

References:

1. Garbers C. & Scheller J., 2013. Interleukin-6 and interkeukin-11: Same same but different. Biol. Chem. 394:1145.
2. Kang S. et al., 2020. Historical overview of the interleukin-6 family cytokine. J. Exp. Med. 217: e20190347.

Figures

Validation of IL-11Rα expression
Validation of IL-11Rα expression

Human IL-11Rα mRNA expression in HEK-Blue™ IL-11 cells.
Total mRNA was extracted from ~1x106 HEK-Blue™ parental cells (blue) and HEK-Blue™ IL-11 cells (red). Human IL-11Rα mRNA was amplified using quantitative RT-qPCR. Data are represented as the log2 fold change comparing hIL-11Rα relative expression between the two cell lines.

Cellular response to IL-11
Cellular response to IL-11

Dose-response of HEK-Blue™ IL-11 cells to recombinant IL-11.
Cells were stimulated with increasing concentrations of recombinant human IL-11 (hIL-11) and murine IL-11 (mIL-11). After overnight incubation, the STAT3 response was determined using QUANTI‑Blue™ Solution, a SEAP detection reagent. The optical density (OD) at 630 nm is shown as mean ± SEM.

Cell line specificity
Cell line specificity

Response of HEK-Blue™ IL-11 cells to a panel of cytokines.
Cells were stimulated with various human and murine recombinant cytokines: 1 ng/ml of hIL-11, mIL-11, or hIL-6, and 10 ng/ml hIL‑27 or TNF-α. After overnight incubation, SEAP activity was assessed using QUANTI‑Blue™ Solution. The optical density (OD) at 630 nm is shown as mean ± SEM.

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Specifications

Antibiotic resistance: Blasticidinhygromycin, and  Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Specificity: human & murine IL-11

Detection range: 

  • 0.1 - 100 ng/ml for human IL-11
  • 0.1 - 100 ng/ml for murine IL-11

Quality Control:

  • The expression of human IL-11Rα has been confirmed by RT-qPCR.
  • STAT3-dependent SEAP reporter activity in response to human and murine IL-11 has been validated.
  • The stability for 20 passages, following thawing, has been verified. 
  • These cells are guaranteed mycoplasma-free. 

This product is covered by a Limited Use License (See Terms and Conditions).

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Contents

  • 3-7 x 106 HEK-Blue™ IL-11 cells in a cryovial or shipping flask
  • 1 ml Normocin™ (50 mg/ml)
  • 2 x 1 ml of HEK-Blue Selection (250X)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent).

Shipped on dry ice (Europe, USA & Canada)

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Details

The cytokine interleukin 11 (IL-11) belongs to the IL-6 family,  which also includes IL-6, IL-27 p28/IL-30, IL-31, Leukemia inhibitory factor (LIF), Oncostatin M (OSM), Cardiotrophin-like cytokine (CLC), Ciliary neurotrophic factor (CNTF), Cardiotrophin-1 (CT-1), and Neuropoietin [1-3]. IL-11 has pleiotropic physiological functions, ranging from development and tissue homeostasis, to immune responses [1, 2]. IL-11 signalisation requires a multimeric structure comprising two specific α chains (IL-11Rα), two gp130 chains (common to the IL-6 signaling receptor), and 2 cytokines. This hexameric complex allows the juxtaposition of the intracellular Janus kinases (JAK) and activation of the signal transducer and activator of transcription 3 (STAT3) [1,3]. Other signaling pathways include the Ras-MAPK pathway, the PI3-K-Akt pathway, and the p38 and JNK MAPK pathways [2, 3].

While gp130 is ubiquitously expressed, IL-11Rα expression is cell-specific. Soluble IL-11Rα resulting from IL-11R proteolysis allows trans-signaling and widens the number of target cells [4]. Importantly, IL-11, like IL-6, has no affinity toward gp130 in the absence of its specific receptor α chain [1]. 

 

 

1. Garbers C. & Scheller J., 2013. Interleukin-6 and interkeukin-11: Same same but different. Biol. Chem. 394:1145.
2. Kang S. et al., 2020. Historical overview of the interleukin-6 family cytokine. J. Exp. Med. 217: e20190347.
3. Morris R. et al., 2018. The molecular details of cytokine signaling via the JAK/STAT pathway. Protein Science. 27:1984.
4. Lamertz L. et al., 2018. Soluble gp130 prevents interleukin-6 and interleukin-11 cluster signaling but not intracellular autocrine responses. Science Sign. 11(550):eaar7388).

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FAQ

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