IL-11 Reporter HEK 293 Cells
HEK-Blue™ IL-11 Cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Human IL-11 Reporter Cells |
3-7 x 10e6 cells |
hkb-hil11r |
You may also need : HEK-Blue™ Selection | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
HEK-Blue™ IL-11 Cells signaling pathway
Interleukin-11 Reporter Cells
HEK-Blue™ IL-11 cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive interleukin-11 (IL-11) by monitoring the activation of the JAK/STAT3 pathway. IL-11 has pleiotropic physiological functions, ranging from development and tissue homeostasis, to immune responses [1, 2].
Cell line description:
HEK-Blue™ IL-11 cells were generated by stable overexpression of the genes encoding the alpha chain of human IL-11 receptor (IL-11Rα), human STAT3, and a STAT3-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. STAT3-dependent SEAP activity is readily assessable in the supernatant using QUANTI-Blue™ Solution, a detection reagent.
Of note, HEK-Blue™ IL-11 cells also respond to human IL-6 and IL-27 (see Figures) as they endogenously express the gp130 co-receptor shared by IL-11 and other cytokines of the IL-6 family.
Features of HEK-Blue™ IL-11 cells:
- Fully functional IL-11 signaling pathway
- Readily assessable STAT3-inducible SEAP reporter activity
- The stability for 20 passages has been verified
- Functionally tested and guaranteed mycoplasma-free
Applications of HEK-Blue™ IL-11 cells:
- Detection of human IL-11 (0.1 - 100 ng/ml) and murine IL-11 (0.1 -100 ng/ml)
- Screening of anti-IL-11 and anti-IL-11Rα antibodies
References:
1. Garbers C. & Scheller J., 2013. Interleukin-6 and interkeukin-11: Same same but different. Biol. Chem. 394:1145.
2. Kang S. et al., 2020. Historical overview of the interleukin-6 family cytokine. J. Exp. Med. 217: e20190347.
Specifications
Antibiotic resistance: Blasticidin, hygromycin, and Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Specificity: human & murine IL-11
Detection range:
- 0.1 - 100 ng/ml for human IL-11
- 0.1 - 100 ng/ml for murine IL-11
Quality Control:
- The expression of human IL-11Rα has been confirmed by RT-qPCR.
- STAT3-dependent SEAP reporter activity in response to human and murine IL-11 has been validated.
- The stability for 20 passages, following thawing, has been verified.
- These cells are guaranteed mycoplasma-free.
This product is covered by a Limited Use License (See Terms and Conditions).
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- 3-7 x 106 HEK-Blue™ IL-11 cells in a cryovial or shipping flask
- 1 ml Normocin™ (50 mg/ml)
- 2 x 1 ml of HEK-Blue Selection (250X)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent).
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
The cytokine interleukin 11 (IL-11) belongs to the IL-6 family, which also includes IL-6, IL-27 p28/IL-30, IL-31, Leukemia inhibitory factor (LIF), Oncostatin M (OSM), Cardiotrophin-like cytokine (CLC), Ciliary neurotrophic factor (CNTF), Cardiotrophin-1 (CT-1), and Neuropoietin [1-3]. IL-11 has pleiotropic physiological functions, ranging from development and tissue homeostasis, to immune responses [1, 2]. IL-11 signalisation requires a multimeric structure comprising two specific α chains (IL-11Rα), two gp130 chains (common to the IL-6 signaling receptor), and 2 cytokines. This hexameric complex allows the juxtaposition of the intracellular Janus kinases (JAK) and activation of the signal transducer and activator of transcription 3 (STAT3) [1,3]. Other signaling pathways include the Ras-MAPK pathway, the PI3-K-Akt pathway, and the p38 and JNK MAPK pathways [2, 3].
While gp130 is ubiquitously expressed, IL-11Rα expression is cell-specific. Soluble IL-11Rα resulting from IL-11R proteolysis allows trans-signaling and widens the number of target cells [4]. Importantly, IL-11, like IL-6, has no affinity toward gp130 in the absence of its specific receptor α chain [1].
1. Garbers C. & Scheller J., 2013. Interleukin-6 and interkeukin-11: Same same but different. Biol. Chem. 394:1145.
2. Kang S. et al., 2020. Historical overview of the interleukin-6 family cytokine. J. Exp. Med. 217: e20190347.
3. Morris R. et al., 2018. The molecular details of cytokine signaling via the JAK/STAT pathway. Protein Science. 27:1984.
4. Lamertz L. et al., 2018. Soluble gp130 prevents interleukin-6 and interleukin-11 cluster signaling but not intracellular autocrine responses. Science Sign. 11(550):eaar7388).