IL-9 Reporter HEK 293 Cells
HEK-Blue™ IL-9 Cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
HEK 293 reporter cells for human and murine IL-9 cytokines |
3-7 x 10e6 cells |
hkb-il9 |
You may also need : QUANTI-Blue™ | View more associated products ▼
Notification: This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.
HEK-Blue™ IL-9 Cells signaling pathway
Interleukin-9 Reporter Cells
HEK-Blue™ IL-9 cells were engineered from the human embryonic kidney HEK 293 cell line to detect bioactive interleukin-9 (IL-9) by monitoring the activation of the JAK/STAT5 pathway. Originally identified as a growth factor of T cells and mast cells, IL-9 is now regarded as a multifunctional cytokine that regulates the function of many different cell types and plays an important role in immunity and immune pathogenesis [1-3].
Cell line description:
HEK-Blue™ IL-9 cells were generated by stable overexpression of the genes encoding the alpha chain of human IL-9 receptor (IL-9Rα), human IL-2Rγ, human JAK3, human STAT5b, and a STAT5-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. STAT5-dependent SEAP activity is readily assessable in the supernatant using QUANTI-Blue™ Solution, a detection reagent. Of note, HEK-Blue™ IL-9 cells also respond to human type II interferons (IFN-γ; see Figures). However, they do not respond to human type I IFNs (IFN-α/IFN-β), as they are knock-out for IFNAR2.
Features of HEK-Blue™ IL-9 cells:
- Fully functional IL-9 signaling pathway
- Readily assessable STAT5-inducible SEAP reporter activity
- The stability for 20 passages has been verified
- Functionally tested and guaranteed mycoplasma-free
Applications of HEK-Blue™ IL-9 cells:
- Detection of human IL-9 (100 pg/ml - 100 ng/ml) and murine IL-9 (30 pg/ml -100 ng/ml)
- Screening of anti-IL-9 and anti-IL-9R antibodies
References:
1. Ritobrata G. & Kaplan M.H., 2011. A brief history of IL-9. J Immunol. 186(6):3283-8.
2. Chakraborty S. et al., 2019. An update on interleukin-9: From its cellular source and signal transduction to its role in immunopathogenesis. Int J Mol Sci. 20(9):2113.
3. Patrussi L. et al., 2021. Interleukin (IL)-9 supports the tumor-promoting environment of chronic lymphocytic leukemia. Cancers (Basel). 13(24):6301.
Specifications
Antibiotic resistance: Blasticidin, Hygromycin, Puromycin, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Specificity: human and murine IL-9
Detects human and murine IL-9
- Detection range for human IL-9: 100 pg - 100 ng/ml
- Detection range for murine IL-9: 30 pg - 100 ng/ml
This product is covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml HEK-Blue™ Selection (250x concentrate)
- 1 ml of Puromycin (10 mg/ml)
- 1 ml Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
Interleukin 9 (IL-9) is a secreted cytokine produced primarily by T helper 2 (Th2) cells. It supports the growth of activated T cell subsets and promotes mast cell proliferation and differentiation [1, 2]. In addition, IL-9 has a direct effect on epithelial cell function stimulating the production of mucin and T cell chemoattractants [2, 3].
IL-9 signals through the heterodimeric cell surface IL-9 receptor (IL-9R) consisting of IL-9Rα (also called CD129) and IL-2Rγ (also called the common γ-chain or CD132). The binding of IL-9 to its receptor triggers three main signaling pathways: JAK/STAT, MAPK, and PI3K [1, 2]. IL-9 is an interesting therapeutic target as it plays a critical role in immunity to parasitic infections, the pathogenesis of cancer and immuno-pathological conditions such as asthma [5, 6].
1. Ritobrata G. & Kaplan M.H., 2011. A brief history of IL-9. J Immunol. 186(6):3283-8.
2. Chakraborty S. et al., 2019. An update on interleukin-9: From its cellular source and signal transduction to its role in immunopathogenesis. Int J Mol Sci. 20(9):2113.
3. Longphre M. et al., 1999. Allergen-induced IL-9 directly stimulates mucin transcription in respiratory epithelial cells. J Clin Invest . 104(10):1375-82.
4. Little F.F. et al., 2001. Il-9 stimulates release of chemotactic factors from human bronchial epithelial cells.Am J Respir Cell Mol Biol . 25(3):347-52.
5. Silva N.S.L. et al., 2021. Interleukin-9 in immunopathology of Trypanosoma cruzi experimental infection. Front Cell Infect Microbiol. 11:756521.
6. Patrussi L. et al., 2021. Interleukin (IL)-9 supports the tumor-promoting environment of chronic lymphocytic leukemia. Cancers (Basel). 13(24):6301.