IL-33 Reporter HEK 293 Cells
|HEK-Blue™ IL-33 cells||Unit size||Cat. code||Docs||Qty||Price|
Human IL-33 Reporter Cells
3-7 x 10e6 cells
IL-33 Reporter Cells
HEK-Blue™ IL-33 cells are designed to detect bioactive IL-33 by monitoring the activation of the NF-κB and AP-1 pathways.
They were generated by stable transfection of human embryonic kidney HEK293-derived cells with the human IL1RL1 gene. In addition, the TNF-α and the IL-1β responses have been blocked.
Therefore, HEK-Blue™ IL-33 cells respond specifically to IL-33. These cells express an NF-κB/AP-1- inducible SEAP reporter gene.
Binding of human IL-33 to the heterodimeric IL-33 receptor on the surface of these cells triggers a signaling cascade leading to the activation of NF-κB and the subsequent production of SEAP.
Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™ Solution.
FEATURES OF HEK-BLUE™ IL-33 CELLS:
- Fully functional IL-33 signaling pathway
- Do not respond to human TNF-α
- Do not respond to human IL-1β
- Readily assessable SEAP reporter activity
- Functionally tested and guaranteed mycoplasma-free
APPLICATIONS OF HEK-BLUE™ IL-33 CELLS:
- Detection of human IL-33
- Screening of anti-IL-33 antibodies
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Specificity: Detects human IL-33
Detection range: 0.5 - 100 ng/mlBack to the top
- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml of HEK-Blue™ Selection (250x concentrate)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA & Canada)Back to the top
Interleukin-33 (IL-33) is a member of the IL-1 family, a group of cytokines that play important roles in host defense, immune regulation and inflammation.
IL-33 mediates its biological effects through IL1RL1 (also known as ST2), a receptor expressed on Th2 and mast cells.
IL-33 and IL1RL1 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex.
IL-33 signaling leads to the activation of NF-kB and MAP kinases, and the production of Th2-associated cytokines.Back to the top