HEK-Blue™ IL-33 Cells

IL-33 Reporter Cells

HEK-Blue™ IL-33 cells are designed to detect bioactive IL-33 by monitoring the activation of the NF-κB and AP-1 pathways.

They were generated by stable transfection of HEK293-derived cells with the human IL1RL1 gene. In addition, the TNF-α and the IL-1β responses have been blocked.

Therefore, HEK-Blue™ IL-33 cells respond specifically to IL-33. These cells express an NF-κB/AP-1- inducible SEAP reporter gene.

Binding of human IL-33 to the heterodimeric IL-33 receptor on the surface of these cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.

Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.

Specifications

Antibiotic resistance: blasticidin, hygromycin B, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Specificity: Detects human IL-33

Detection range: 0.5 - 100 ng/ml

Contents

• 1 vial containing 3-7 x 10⁶ cells.
• 2 x 1 ml HEK-Blue™ Selection (250X concentrate); a solution containing several selection antibiotics.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).

Shipped on dry ice (Europe, USA & Canada)

Description

Interleukin-33 (IL-33) is a member of the IL-1 family, a group of cytokines that play important roles in host defense, immune regulation and inflammation.

IL-33 mediates its biological effects through IL1RL1 (also known as ST2), a receptor expressed on Th2 and mast cells.

IL-33 and IL1RL1 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex.

IL-33 signaling leads to the activation of NF-kB and MAP kinases, and the production of Th2-associated cytokines.