HEK-Blue™ IL-33 Cells
|HEK-Blue™ IL-33 cells||Unit size||Cat. code||Docs||Qty||Price|
3-7 x 10e6 cells
IL-33 Sensor Cells
HEK-Blue™ IL-33 cells are designed to detect bioactive IL-33 by monitoring the activation of the NF-κB and AP-1 pathways. They were generated by stable transfection of HEK293-derived cells with the human IL1RL1 gene. In addition, the TNF-α and the IL-1β responses have been blocked. Therefore, HEK-Blue™ IL-33 cells respond specifically to IL-33. These cells express an NF-κB/AP-1- inducible SEAP reporter gene.
Binding of human IL-33 to the heterodimeric IL-33 receptor on the surface of these cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP. Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.
EK-Blue™ IL-33/IL-1β cells were incubated with human IL-33, IL-1β, IL-18 and TNF-α. After a 24h incubation, SEAP activity was assessed using QUANTI-Blue™ and reading optical density (O.D.) at 655 nm.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum (30 min at 56°C), 50 U/ml penicillin, 50 μg/ml streptomycin, 100 μg/ml Normocin™
Shipped on dry ice
Specificity: Detects human IL-33
Detection range: 0.5 - 100 ng/ml
• 1 vial containing 3-7 x 106 cells.
• 2 x 1 ml HEK-Blue™ Selection (250X concentrate); a solution containing several selection antibiotics.
• 1 ml Normocin™ (50 mg/ml). Normocin™ is a formulation of three antibiotics active against mycoplasmas, bacteria and fungi.
• 1 pouch of QUANTI-Blue™ (SEAP detection medium).Back to the top
Interleukin-33 (IL-33) is a member of the IL-1 family, a group of cytokines that play important roles in host defense, immune regulation and inflammation. IL-33 mediates its biological effects through IL1RL1 (also known as ST2), a receptor expressed on Th2 and mast cells.
IL-33 and IL1RL1 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex. IL-33 signaling leads to the activation of NF-kB and MAP kinases, and the production of Th2-associated cytokines.Back to the top