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HEK-Blue™ IL-33 Cells

HEK-Blue™ IL-33 cells Unit size Cat. code Docs Qty Price
Human IL-33 Reporter Cells
3-7 x 10e6 cells
hkb-hil33
+-
$1,182.00

August 21st 2019 - CONTENTS UPDATE NOTIFICATION
Please note that, for your convenience, these cells are now provided with QUANTI-Blue™ Solution.

IL-33 Reporter Cells

HEK-Blue™ IL-33 cells are designed to detect bioactive IL-33 by monitoring the activation of the NF-κB and AP-1 pathways.

They were generated by stable transfection of HEK293-derived cells with the human IL1RL1 gene. In addition, the TNF-α and the IL-1β responses have been blocked.

Therefore, HEK-Blue™ IL-33 cells respond specifically to IL-33. These cells express an NF-κB/AP-1- inducible SEAP reporter gene.

Binding of human IL-33 to the heterodimeric IL-33 receptor on the surface of these cells triggers a signaling cascade leading to the activation NF-κB and the subsequent production of SEAP.

Levels of SEAP in the supernatant can be easily determined with QUANTI-Blue™.

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Specifications

Antibiotic resistance: blasticidin, hygromycin B, Zeocin™

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free

Specificity: Detects human IL-33

Detection range: 0.5 - 100 ng/ml

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Contents

  • 1 vial containing 3-7 x 106 cells
  • 2 x 1 ml of HEK-Blue™ Selection (250x concentrate)
  • 1 ml of Normocin™ (50 mg/ml)
  • 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)

Shipped on dry ice (Europe, USA & Canada)

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Description

HEK-Blue IL-33/IL-1beta cells pathway | InvivoGen

Interleukin-33 (IL-33) is a member of the IL-1 family, a group of cytokines that play important roles in host defense, immune regulation and inflammation.

IL-33 mediates its biological effects through IL1RL1 (also known as ST2), a receptor expressed on Th2 and mast cells.

IL-33 and IL1RL1 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex.

IL-33 signaling leads to the activation of NF-kB and MAP kinases, and the production of Th2-associated cytokines.

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Citations

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