Anti-hIL-33 Neutralizing mAb

Neutralizing monoclonal antibody against human interleukin 33

Neutralizing monoclonal antibody against human IL-33

Anti-hIL-33-IgG (clone 19G8) is a monoclonal antibody (mAb) specific for human interleukin 33 (hIL-33). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-33. IL-33 is a pro-inflammatory cytokine that shares structural and functional characteristics with the IL-1 cytokine family. It binds and signals through the IL-1RL1/ IL-1R accessory protein (IL-1RAcP) receptor activating NF-κB and MAP kinases.

More details

Key features:

• A potent and specific neutralizing mAb against hIL-33
• Provided azide-free
• Each lot is functionally tested

References:

1. Arend W. et al., 2008. IL-1, IL-18, and IL-33 families of cytokines. Immunol Rev. 223:20-38.
2. Schiering C. et al., 2014. The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature. 513(7519):564-8.

Figures

Anti-hIL-33-IgG is a potent neutralization antibody. Increasing concentrations of Anti-hIL-33-IgG were incubated for 30 minutes with the recombinant cytokine hIL-33 (1 ng/ml) prior to the addition of HEK-Blue™ IL-33 cells. After overnight incubation, SEAP activity in the cell culture supernatant was assessed using QUANTI-Blue™ Solution. Data are shown in percentage (%) of neutralization.

Specifications

Target: Natural and recombinant human interleukin 33 (IL-33)

Clone: 19G8

Isotype: Mouse IgG1, kappa

Control: Mouse IgG1 Control

Immunogen: Human IL-33 protein expressed in Swiss mice following DNA immunization

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Tested applications: Blocking & Neutralisation

Quality control:

• This product has been validated for neutralization.
• The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Contents

3 x 100 μg purified anti-hIL-33-IgG antibody, provided azide-free and lyophilized

 The product is shipped at room temperature.

 Upon receipt, store lyophilized antibody at -20 °C.

Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.

 Avoid repeated freeze-thaw cycles.

Details

Interleukin-33 (IL-33; also known as IL-1F11) is a member of the IL-1 family, a group of cytokines that play important roles in host defense, immune regulation, and inflammation [1]. IL-33 mediates its biological effects through  IL1RL1  (also known as ST2), a receptor expressed on Th2 and mast cells. IL-33 and  IL1RL1 form a complex with IL-1R accessory protein (IL-1RAcP), a signaling receptor subunit that is also a member of the IL-1R complex. IL-33 signaling leads to the activation of NF-κB and MAP kinases.

IL-33 can function both as a traditional cytokine and as a nuclear factor regulating gene transcription. Following pro-inflammatory stimulation, Il-33 can induce Th2-biased immune responses, such as the production of IL-4, IL-5 and IL-13 [2]. In addition, as IL-33 is constitutively expressed in endothelial and epithelial cells, it can act as an endogenous danger signal, or damage-associated molecular pattern (DAMP; also called alarmins), in response to tissue damage [3, 4].

1. Arend W. et al., 2008. IL-1, IL-18, and IL-33 families of cytokines. Immunol Rev. 223:20-38.
2. Schiering C. et al., 2014. The alarmin IL-33 promotes regulatory T-cell function in the intestine. Nature. 513(7519):564-8.
3. Cayrol C. & Girard JP., 2014. IL-33: an alarmin cytokine with crucial roles in innate immunity, inflammation and allergy. Curr Opin Immunol. 31-7.
4. Oboki K. et al., 2011. IL-33 and airway inflammation. Allergy Asthma Immunol Res. 3(2): 81–88