IL-6 Reporter HEK 293 Cells
HEK-Blue™ IL-6 cells | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Human IL-6 Reporter Cells |
3-7 x 10e6 cells |
hkb-hil6 |
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IL-6 Reporter Cells
HEK-Blue™ IL-6 Cells signaling pathway
HEK-Blue™ IL-6 cells allow the detection of bioactive human interleukin-6 (IL-6) by monitoring the activation of the STAT-3 pathway. The proinflammatory cytokine IL-6 is one of the most important mediators of fever and of the acute phase response.
More details on proinflammatory cytokine IL-6
Cell line description:
HEK-Blue™ IL-6 cells were generated by stable transfection of human embryonic kidney 293 (HEK293)‑derived cells with the genes encoding human IL-6 receptor (IL-6R) and signal transducer and activator of transcription 3 (STAT3). In order to detect the activation of the IL-6 pathway, they were further transfected with a STAT3-inducible secreted embryonic alkaline phosphatase (SEAP) reporter gene.
Upon IL-6 stimulation, HEK-Blue™ IL-6 cells trigger the activation of STAT3 and the subsequent secretion of SEAP. Levels of STAT3-induced SEAP can be readily monitored using QUANTI-Blue™ Solution.
Stimulation of HEK-Blue™ IL-6 cells with recombinant human IL-6 can be blocked by anti-hIL-6-IgG.
Features of HEK-Blue™ IL-6 cells:
- Fully functional IL-6 signaling pathway
- Readily assessable SEAP reporter activity
- The stability for 20 passages has been validated
- Functionally tested and guaranteed mycoplasma-free
Applications of HEK-Blue™ IL-6 cells:
- Detection of human IL-6
- Screening of anti-IL-6 or anti-IL-6R antibodies
- Screening of JAK1/JAK2 inhibitors
Specifications
Antibiotic resistance: blasticidin, hygromycin, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Specificity: human IL-6
Detection range: 0.03 - 10 ng/ml for human IL-6
This product is covered by a Limited Use License (See Terms and Conditions).
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- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml of HEK-Blue™ Selection (250x concentrate)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
The proinflammatory cytokine IL-6 is one of the most important mediators of fever and of the acute phase response [1, 2].
IL-6 exerts its action by first binding to the IL-6R. The complex of IL-6 and IL-6R associates with the signal-transducing membrane protein gp130, thereby inducing its dimerization.
This leads to the activation by phosphorylation of the tyrosine kinases of the Janus family (JAK1, JAK2, and Tyk2).
Activated JAKs induce dimerization and translocation to the nucleus of STAT3 where it binds enhancer elements of IL-6-inducible genes [3].
In HEK-Blue™ IL-6 cells activation of the JAK/STAT3 pathway with IL-6 leads to the production of SEAP.
1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.
3. Murray P., 2007. The JAK-STAT Signaling Pathway: Input and Output Integration. J. Immunol. 178:2623-9.