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Anti-hIL-6 Neutralizing mAb

Anti-hIL-6-IgG Unit size Cat. code Docs Qty Price
Human IL-6 Neutralizing Antibody (clone 3H3) - Monoclonal Mouse IgG1
3 x 100 µg
mabg-hil6-3
+-
$285.00

Neutralizing monoclonal antibody against human interleukin 6

Neutralizing monoclonal antibody against human IL-6
Neutralizing monoclonal antibody against human IL-6

Anti-hIL-6-IgG (clone 3H3) is a monoclonal antibody (mAb) specific for human interleukin 6 (hIL-6). It is produced in hybridomas and purified by affinity chromatography. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIL-6. IL-6 is a multifunctional cytokine that plays an important role in the regulation of the immune response, hematopoiesis, and inflammation [1, 2].

More details More details

 

Key features:

  • A potent and specific neutralizing mAb against hIL-6
  • Provided azide-free
  • No cross-reactivity with murine IL-6
  • Each lot is functionally tested

 

References:

1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.

Figures

Evaluation of IL-6 inhibition
Evaluation of IL-6 inhibition

Dose-dependent inhibition of IL-6 response using Anti-hIL-6-IgG neutralizing antibody in HEK-Blue™ IL-6 cells. Anti-hIL-6-IgG was incubated with 1 ng/ml of hIL-6 for 30 minutes prior to the addition of HEK‑Blue™ IL-6 cells. After overnight incubation, SEAP activity was assessed using QUANTI-Blue™ Solution. Data represent % inhibition of reporter activity.

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Specifications

Target: Natural and recombinant human interleukin 6 (IL-6)

Specificity: No cross-reactivity with mouse IL-6

Clone: 3H3

Isotype: Mouse IgG1, kappa

Control: Mouse IgG1 Control

Immunogen: Human IL-6 protein expressed in Swiss mice following DNA immunization

Formulation: 0.2 µm filtered solution in a sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Tested applications: Blocking & Neutralisation

Quality control:

  • This product has been validated for neutralization.
  • The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.
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Contents

  • 3 x 100 μg purified anti-hIL-6-IgG antibody, provided azide-free and lyophilized.

 

room temperature The product is shipped at room temperature.

store Upon receipt, store lyophilized antibody at -20 °C.

stable Reconstituted antibody is stable for 1 month at 4 °C and for 1 year at -20 °C.

Alert Avoid repeated freeze-thaw cycles.

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Details

The proinflammatory cytokine interleukin 6 (IL-6) is one of the most important mediators of fever and of the acute phase response [1, 2]. IL-6 exerts its action by first binding to the IL-6 receptor (IL-6R). The complex of IL-6 and IL-6R associates with the signal-transducing membrane protein gp130, thereby inducing its dimerization.
This leads to the activation by phosphorylation of the tyrosine kinases of the Janus family (JAK1, JAK2, and Tyk2). Activated JAKs induce dimerization and translocation to the nucleus of STAT3 where it binds enhancer elements of IL-6-inducible genes [3].
In HEK-Blue™ IL-6 cells activation of the JAK/STAT3 pathway with IL-6 leads to the production of the secreted alkaline phosphatase (SEAP) reporter.

 

1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Akira S. et al., 1990. Biology of multifunctional cytokines: IL 6 and related molecules (IL 1 and TNF). FASEB J. 4(11):2860-7.
3. Murray P., 2007. The JAK-STAT Signaling Pathway: Input and Output Integration. J. Immunol. 178:2623-9.

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