Human IL-6 Antibody

Mouse IgG1 (clone 3H3) - Recombinant

ABOUT

Recombinant anti-human IL-6 neutralizing monoclonal antibody

Anti-hIL-6-mIgG1 is a monoclonal antibody (mAb) targeting human interleukin-6 (hIL-6).

It was previously extracted from hybridoma cells (clone 3H3). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. This antibody has been selected for its ability to neutralize the biological activity of hIL-6 efficiently.

IL-6 is a multifunctional cytokine that plays an important role in the regulation of the immune response, hematopoiesis, and inflammation.

More details More details

This antibody can be used together with HEK-Blue™ IL-6 Cells treated with recombinant human IL-6 for screening and neutralization assays to block interleukin-6 signaling.

 

Key features

  • Each lot is functionally tested and validated.
  • The complete sequence of the antibody construct has been verified.

  • The absence of endotoxins is determined by the EndotoxDetect™ assay.

     

The hybridoma-derived Anti-hIL-6-IgG (mabg-hil6-3) antibody has been replaced by Anti-hIL-6-mIgG1 (mab9-hil6), which is produced by recombinant technology and purified from CHO cells.

Disclaimer:  These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.

Binding of Anti-hIL-6 mAb
Binding of Anti-hIL-6 mAb

SPECIFICATIONS

Specifications

Target

IL-6

Target species

Human

Applications

Neutralization (tested)

ELISA

Antibody applications
Neutralization assay
Species
Human
Isotype
mIgG1
kappa
Recommended isotype control
Recombinant Mouse IgG1 Isotype Control Antibody
Clone
C5-3H3
Tag
Tag-free
Source
CHO cells
Production details
Animal-free
Purification
Protein G
Formulation buffer

Sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Purity
≥ 95% (SDS-PAGE)
Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Quality control

Each lot is released only after meeting predefined biological activity specifications in a cell-based assay.

CONTENTS

Contents

  • Product: 
    Anti-hIL-6-mIgG1
  • Cat code: 
    mab9-hil6
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

The proinflammatory cytokine IL-6 is one of the most important mediators in the regulation of the immune response, hematopoiesis, and inflammation [1]. It plays roles in chronic inflammation, autoimmune diseases, and cancer. In addition, it has been reported to be involved in the cytokine storm triggered by the corona virus disease 2019 (COVID-19) [2].
 

IL-6 exerts its action by first binding to the IL-6 receptor (IL-6R). The complex of IL-6 and IL-6R associates with the signal-transducing membrane protein gp130, thereby inducing its dimerization. This leads to the downstream activation by phosphorylation of certain tyrosine kinases within the Janus family (JAK1, JAK2, and Tyk2). Subsequently, activated JAKs induce phosphorylation, dimerization and translocation of the signal transducer and activator of transcription 3 (STAT3) to the nucleus where it binds enhancer elements of IL-6 inducible genes [3]. Considering the various properties of this pleiotropic cytokine, the IL-6–STAT3 signaling axis is now considered a major target for the treatment of inflammatory diseases [2].

 

References:

1. Kang S. et al., 2015. Therapeutic uses of anti-interleukin-6 receptor antibody. Int. Immunol. 27: 21-9.
2. Hirano T., 2021. IL-6 in inflammation, autoimmunity and cancer. Int Immunol. 1;33(3):127-148.
3. Murray P., 2007. The JAK-STAT Signaling Pathway: Input and Output Integration. J. Immunol. 178:2623-9.

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