Recombinant human IL-6
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Recombinant Cytokine, source: E. coli
Recombinant human interleukin-6 (with HSA)
Recombinant human interleukin 6 (IL-6) is a single, nonglycosylated, polypeptide chain containing 185 amino acids. IL-6 has a molecular weight of 21kDa. IL-6 is produced in Escherichia coli.
IL-6 is used to induce its cognate signalling pathway in HEK-Blue™ IL-6.Back to the top
Source: E. coli
UniProt ID: P05231
Formulation: Lyophilized from a 0.2 µm filtered phosphate buffer solution (pH 7.4) containing human serum albumin (HSA).
Purity: >95%, by SDS-PAGE.
Molecular mass: ~ 21 kDa
Solubility: 100 μg/ml in water.
Endotoxin level: Less than 0.1 EU/µg as determined by the LAL method.
Bioactivity: Validated using HEK-Blue IL6 cells.
Specific activity: IL-6 is fully biologically active when compared to standard. The ED50 as determined by the dose-dependant stimulation of human TF-1 cells is less than 0.1 ng/ml, corresponding to a Specific Activity of 5 x 107 IU/mg.Back to the top
- 10 μg of IL-6 provided as a white lyophilized powder.
- 1.5 ml endotoxin-free water.
IL-6 is shipped at room temperature.
Upon receipt it should be stored at -20°C.Back to the top