IL-4 & IL-13 Reporter HEK 293 Cells
Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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HEK-Blue™ IL-4/IL-13 cells Human IL-4 / IL-13 SEAP Reporter Cells |
Show product |
3-7 x 10e6 cells |
hkb-il413
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HEK-Blue™ IL-4/IL-13 vial Additional cell vial |
Show product |
3-7 x 10e6 cells |
hkb-il413-av
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Notification: Reference #hkb-il413-av can only be ordered together with reference #hkb-il413.
IL-4 & IL-13 Reporter Cells
HEK-Blue™ IL-4/IL-13 Cells signaling pathway
HEK-Blue™ IL-4/IL-13 cells allow the detection of bioactive human interleukin-4 (IL-4) and human/murine IL-13 by monitoring the activation of the STAT6 pathway. IL-4 and IL-13 are cytokines that play an important role in regulating inflammation and immune responses [1].
Cell line description:
HEK-Blue™ IL-4/IL-13 cells were generated by stable transfection with the gene encoding human STAT6 to obtain a fully active STAT6 pathway. The other genes of the IL-4/IL-13 pathway are endogenously expressed. These cells were further transfected with a STAT6-inducible secreted embryonic alkaline phosphatase (SEAP) reporter. The binding of IL-4 or IL-13 to their receptor triggers a signaling cascade leading to the activation of STAT6 and the subsequent production of SEAP. This can be readily assessed in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent.
HEK-Blue™ IL-4/IL-13 cells detect human (h) IL-4 and hIL-13, as well as murine (m) IL-13 (see figures). Stimulation of HEK-Blue™ IL-4/IL-13 cells with recombinant human IL-4 or IL-13 can be blocked by the neutralizing monoclonal hIL-4 and hIL-13-IgG antibodies, respectively.
Key features:
- Fully functional IL-4/IL-13 signaling pathway
- Readily assessable STAT6-SEAP reporter activity
Applications:
- Detection of human IL-4 and human/murine IL-13
- Screening of anti-IL-4, anti-IL4Rα, anti-IL-13, or anti-IL-13Rα1 antibodies
Reference:
1. Kasaian MT. et al., 2013. An IL-4/IL-13 dual antagonist reduces lung inflammation, airway hyperresponsiveness, and IgE production in mice. Am J Respir Cell Mol Biol.49(1):37-46.
Back to the topSpecifications
Antibiotic resistance: blasticidin, Zeocin®
Growth medium: DMEM, 4.5 g/l glucose, 2-4 mM L-glutamine, 10% (v/v) heat-inactivated fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™
Guaranteed mycoplasma-free
Detects human IL-4 and human/murine IL-13
- Detection range for human IL-4: 0.1 - 10 ng/ml
- Detection range for human IL-13: 1 - 100 ng/ml
This product is covered by a Limited Use License (See Terms and Conditions).
Back to the topContents
- 1 vial containing 3-7 x 106 cells
- 1 ml of Blasticidin (10 mg/ml)
- 1 ml of Zeocin® (100 mg/ml)
- 1 ml of Normocin™ (50 mg/ml)
- 1 ml of QB reagent and 1 ml of QB buffer (sufficient to prepare 100 ml of QUANTI-Blue™ Solution, a SEAP detection reagent)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
Details
The transcription factor STAT6 is activated primarily by two cytokines with overlapping biologic functions, IL-4 and IL-13 [1, 2].
It can also be activated by IFN-α in a cell-specific manner. In non-hematopoietic cells, IL-4 and IL-13 bind a receptor complex composed of the IL-4Ralpha and IL-13Ralpha1.
Upon ligand binding, the receptor complex activates the receptor-associated Janus kinases (JAK1 and Tyk2) leading to the recruitment of STAT6 and its phosphorylation.
Activated STAT6 forms homodimers that translocate to the nucleus where they bind the promoter of responsive genes inducing gene transcription.
In HEK-Blue™ IL-4/IL-13 cells stimulation with IL-4 or IL-13 activates the JAK/STAT6 pathway with the subsequent production of SEAP.
1. Kasaian MT. et al., 2013. An IL-4/IL-13 dual antagonist reduces lung inflammation, airway hyperresponsiveness, and IgE production in mice. Am J Respir Cell Mol Biol.49(1):37-46.
2. David M. et al., 2003. Functional characterization of IL-13 receptor a2 gene promoter: a critical role of the transcription factor STAT6 for regulated expression. Oncogene 22, 3386–3394.