Recombinant human IL-4
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Recombinant Cytokine, source: E. coli
Recombinant human interleukin-4 (with HSA)
Interleukin-4 (IL-4) is a cytokine that plays an important role in regulating inflammation and immune responses. It induces the differentiation of naïve helper T cells to Th2 cells. This cytokine binds the IL-4 receptor that also binds another cytokine interleukin 13 (IL-13), which may explain the overlapping functions of IL-4 and IL-13 [1,2]. Recombinant human IL-4 is intended for use in cell culture applications. It is produced in E. coli.
IL-4 is used to induce its cognate signalling pathway in HEK-Blue™ IL-4/IL-13.
1. Kasaian MT. et al., 2013. An IL-4/IL-13 dual antagonist reduces lung inflammation, airway hyperresponsiveness, and IgE production in mice. Am J Respir Cell Mol Biol.49(1):37-46.
2. David M. et al., 2003. Functional characterization of IL-13 receptor a2 gene promoter: a critical role of the transcription factor STAT6 for regulated expression. Oncogene 22, 3386–3394.
Source: E. coli
UniProt ID: P05112
Formulation: recombinant human IL-4 was lyophilized from a 0.2 µM phosphate buffer solution (pH 7.4) containing human serum albumin (HSA).
Purity: >98%, by HPLC and SDS-PAGE.
Molecular mass: ~ 15 kDa
Solubility: 100 μg/ml in water
Endotoxin level: < 0.1 EU/µg as determined by LAL assay
Working Concentration: 0.5 - 100 ng/ml
Bioactivity: Validated using TF-1 cells.
Specific activity: The ED50 as determined by a cell proliferation assay using human TF-1 cells is less than 0.2 ng/ml, corresponding to a specific activity of >5.0 x 106 IU/mg.Back to the top
- 10 μg of IL-4 provided as a white lyophilized powder.
- 1.5 ml endotoxin-free water.
IL-4 is shipped at room temperature.
Upon receipt it should be stored at -20°C.Back to the top