Recombinant human IL-12
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Recombinant Cytokine, source: CHO
Recombinant human interleukin-12 (with HSA)
Interleukin-12 (IL-12), also known as IL-12p70, cytotoxic lymphocyte maturation factor (CLMF) and natural killer cell stimulatory factor (NKSF), is primarily produced by dendritic cells and macrophages in response to bacterial pathogens [1,2]. It promotes Th1 responses and is a powerful inducer of interferon gamma (IFN-γ). IL-12 is a heterodimeric 70 kDa cytokine composed of two subunits, p35 and p40.
Recombinant human IL-12 is produced in Chinese hamster ovary (CHO) cells transfected with a fusion gene encoding the p35 and p40 subunits linked by a decapeptide containing two elastin motifs. These transfected cells produce and secrete biologically active IL-12. The recombinant protein is purified by size exclusion chromatography.
The activity of recombinant human IL-12 can be monitored in HEK-Blue™ IL-12 cells which express a STAT4-inducible SEAP reporter gene.
1. Vignali DA. & Kuchroo VK., 2012. IL-12 family cytokines: immunological playmakers. Nat Immunol. 13(8):722-8.
2. Trinchieri G.,1995. Interleukin-12: a proinflammatory cytokine with immunoregulatory functions that bridge innate resistance and antigen-specific adaptive immunity. Annu Rev Immunol. 13:251-76.
Source: Mammalian; Chinese hamster ovary (CHO) cells
UniProt ID: P29459
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.4) with human serum albumin (HSA) as a carrier protein
Purity: >90%, by SDS-PAGE
Molecular mass: ~ 70 kDa, by SDS-PAGE
Solubility: 100 μg/ml in water
Endotoxin level: < 0.1 EU/μg protein as determined by the LAL method
Bioactivity: Validated using HEK-Blue IL-12 cellsBack to the top
- 10 μg of recombinant human IL-12 (with HSA) provided as a white lyophilized powder.
- 1.5 ml endotoxin-free water.
Recombinant human IL-12 is shipped at room temperature.
Upon receipt it should be stored at -20°C.Back to the top