Recombinant human IL-18
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Recombinant Cytokine, source: E. coli
Recombinant Human Interleukin-18 (with HSA)
Interleukin 18 (IL-18; formerly called interferon-g inducing factor) is a pro-inflammatory cytokine that causes a variety of biological effects associated with infection and inflammation [1, 2]. More specifically, IL-18 induces IFN-γ production and contributes to T-helper 1 (Th1) cell polarization.
IL-18 is produced by macrophages and other cells, as a proprotein which is proteolytically processed to its active form by caspase 1, an enzyme that is activated within the inflammasome multiprotein complex. IL-18 binds to an heterodimeric receptor consisting of IL-18R and IL-18 receptor accessory protein, leading to the activation of NF-kB and AP-1 .
Recombinant human IL-18 is a 157 amino acid protein with a molecular mass of 18 kDa that is produced in Escherichia coli. It is used to induce its cognate signalling pathway in HEK-Blue™ IL-18 cells.
1. Arend W. et al., 2008. IL-1, IL-18, and IL-33 families of cytokines. Immunol Rev. 223:20-38.
2. Dinarello CA. et al., 1998. Overview of interleukin-18: more than an interferon-gamma inducing factor.J Leukoc Biol. 63(6):658-64.
3. Gracie JA. et al., 2003. Interleukin-18. J Leukoc Biol.3(2):213-24.
Source: E. coli
Uniprot ID: Q14116
Formulation: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.4) with human serum albumin (HSA) as a carrier protein
Purity: >95%, by SDS-PAGE
Molecular mass: ~ 18.3 kDa
Solubility: 100 μg/ml in water
Endotoxin level: < 0.1 EU/μg protein as determined by the LAL method
Bioactivity: Validated using HEK-Blue IL-18 cellsBack to the top
- 10 μg of recombinant human IL-18 provided as a white lyophilized powder.
- 1.5 ml endotoxin-free water.
Recombinant human IL-18 is shipped at room temperature.
Upon receipt it should be stored at -20 °C.Back to the top