Recombinant human IL-1β protein - Bioactive cytokine

Recombinant cytokine, source: CHO cells

ABOUT

Human IL-1β protein - Mammalian cell-expressed, tag-free, carrier-free

Recombinant human IL-1β is a high-quality and biologically active cytokine, validated using proprietary IL-1β reporter cells. This member of the IL-1/TLR cytokine superfamily is produced in CHO cells to ensure protein glycosylation and bona fide 3D structure.

Recombinant human IL-1β can be used together with HEK-Blue™ IL-1β cells for the screening of inhibitory molecules, such as Nidanilimab, a therapeutic monoclonal antibody targeting IL-1β (see figures).

 

Key features

  • Each lot is validated using HEK-Blue™ IL-1β cells
  • Endotoxin < 0.1 EU/µg
  • 0.2 µm sterile-filtered

Applications

  • IL-1β detection and quantification assays (positive control)
  • Screening and release assays for antibodies blocking IL-1β signaling
  • Screening and release assays for engineered IL-1β proteins

 

Interleukin 1 beta (IL-1β) is a secreted pro-inflammatory cytokine that generates systemic and local responses to infection and injury. Dysregulated production of IL-1β contributes to inflammatory diseases such as cryopyrin-associated periodic syndrome (CAPS), familial mediterranean fever (FMF), and systemic juvenile idiopathic arthritis (SJIA).

More details

All InvivoGen products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Source
CHO cells
Species
Human
Synonyms
Catabolin
Accession sequence

P01584

Protein size
153 a.a. (A117-S269)
Molecular weight
~17 kDa (SDS-PAGE)
Carrier
Carrier-free
Tag
Tag-free
Purity
≥ 95% (SDS-PAGE)
Solubility

100 μg/ml in water

Formulation buffer

Phosphate buffer saline (pH 7.4), 5% saccharose

Appearance (form)
Lyophilized
Reconstitution buffer
Endotoxin-free water (provided)
Sterility

0.2 µm filtration

Endotoxin

The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Applications

Cellular assays (tested), ELISA

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Recombinant human IL-1β (CHO)
  • Cat code: 
    rcyc-hil1b
  • Quantity: 
    10 µg
Notes:

1.5 ml endotoxin-free water

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

IL‑1 is a secreted pro‑inflammatory cytokine that plays a critical role in immune responses and inflammation [1]. It exists in two distinct isoforms, IL-1α and IL-1β, which are produced as pro-proteins by activated macrophages. IL-1β is cleaved by caspase-1 [2] while IL-1α is cleaved by calcium‑activated calpain or caspase-5 (or its murine ortholog caspase-11) [3]. In contrast to pro‑IL‑1β, pro-IL-1α is active, although cleavage dramatically enhances its bioactivity. IL-1β and IL-1α bind to the same receptor, IL-1R1, triggering the formation of the IL-1R1/IL-1R3/MyD88 complex. This induces signaling leading to the activation of the transcription factors NK-κB and AP-1 with the subsequent inflammatory response [4].

 

1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27.
2. Lopez-Castejon G. & Brough D., 2011. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22(4):189-95.
3. Wiggins K.A. et al., 2019. IL-1α cleavage by inflammatory caspases of the noncanonical inflammasome controls the senescence-associated secretory phenotype. Aging Cell. 18(3):e12946.
4. Weber A. et al., 2019. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.

DOCUMENTS

Documents

Recombinant human IL-1β (CHO)

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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