Recombinant human IL-1β

Recombinant human interleukin 1 beta (with HSA)

Interleukin 1 beta (IL-1β) is a secreted pro-inflammatory cytokine that participates in the generation of systemic and local responses to infection and injury. Human IL-1β is produced as a precursor (269 amino acids) that is cleaved intracellularly by the cysteine protease Caspase-1 to generate the active cytokine.

Recombinant human interleukin 1 beta (IL-1β) is a non-glycosylated polypeptide chain containing 153 amino acids with a molecular mass of 17000 Daltons. This recombinant cytokine is produced in Escherichia coli.

IL-1β is used to induce its cognate signalling pathway in HEK-Blue™ IL-1β cells.

Specifications

Source: Amino acids Ala117-Ser269 expressed in E. coli

Accession #: NP_000576

Formulation: Lyophilized from a 0.2 μm filtered solution in PBS (pH 7.4) with human serum albumin (HSA) as a carrier protein

Purity: >95%, by SDS-PAGE

Molecular mass: ~ 17 kDa

Solubility: 100 μg/ml in water

Endotoxin level: < 0.1 EU/μg protein as determined by the LAL method

Bioactivity: Validated using HEK-Blue IL-1β cells

Contents

• 10 μg of recombinant human IL-1β (with HSA) provided as a white lyophilized powder.
• 1.5 ml endotoxin-free water.

Recombinant human IL-1β is shipped at room temperature.

Upon receipt it should be stored at -20°C.