Recombinant human IL-1β (E. coli)
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Cat.code:
rcyec-hil1b
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ABOUT
Human IL-1β protein - E. coli -expressed, tag-free, with HSA
Recombinant human IL-1β is a high-quality and biologically active cytokine, validated using proprietary IL-1β reporter cells. This member of the IL-1/TLR cytokine superfamily is produced in E. coli and thoroughly purified to remove endotoxins.
Recombinant human IL-1β can be used together with HEK-Blue™ IL-1β cells for the screening of inhibitory molecules, such as Canakinumab, a therapeutic monoclonal antibody targeting IL-1β (see figures).
Key features
- Each lot is validated using HEK-Blue™ IL-1β cells
- Endotoxin < 0.01 EU/µg
- 0.2 µm sterile-filtered
Applications
- IL-1β detection and quantification assays (positive control)
- Screening and release assays for antibodies blocking IL-1β signaling
- Screening and release assays for engineered IL-1β proteins
Interleukin 1 beta (IL-1β) is a secreted pro-inflammatory cytokine that generates systemic and local responses to infection and injury. Dysregulated production of IL-1β contributes to inflammatory diseases such as cryopyrin-associated periodic syndrome (CAPS), familial mediterranean fever (FMF), and systemic juvenile idiopathic arthritis (SJIA).
All products are for internal research use only, and not for human or veterinary use.
Meet the MiniKines™: Collectible Cytokine Cards
SPECIFICATIONS
Specifications
P01584
100 μg/ml in water
Phosphate buffer saline (pH 7.4), 5% saccharose, 2% HSA
≤ 0.1 EU/μg (measurement by kinetic chromogenic LAL assay)
Cellular assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Recombinant human IL-1β (E. coli)
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Cat code:rcyec-hil1b
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Quantity:10 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Interleukin 1β (IL-1β) is a soluble pro-inflammatory cytokine that plays a critical role in the host response to infection and injury [1]. It is synthesized as a pro-IL-1β zymogen by activated macrophages and must be cleaved by caspase-1 to generate its mature form [2]. IL-1β binding to the IL-1R1 receptor triggers the formation of the IL-1R1/IL-1R3/MyD88 complex and induces signaling leading to the activation of the transcription factors NK-κB and AP-1 [3].
Due to its role in mediating acute and chronic inflammation, IL-1β has emerged as a therapeutic target for auto-inflammatory diseases [1,4].
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8–27.
2. Lopez-Castejon G. & Brough D., 2011. Understanding the mechanism of IL-1β secretion. Cytokine Growth Factor Rev. 22(4):189-95.
3. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.
4. Dinarello CA., 2011. Interleukin-1 in the pathogenesis and treatment of inflammatory diseases. Blood. 117:3720–3732.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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