Anti-hIL-1β-mIgG1
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Cat.code:
mab9-hil1b
- Documents
ABOUT
Recombinant anti-IL-1β neutralizing monoclonal antibody
Anti-hIL-1β-mIgG1 is a monoclonal antibody (mAb) targeting human interleukin-1 beta (hIL-1β).
It was previously extracted from hybridoma cells (clone 4H5). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. This antibody has been selected for its ability to neutralize the biological activity of hIL-1β efficiently.
Interleukin-1 beta (IL-1β) is a soluble pro-inflammatory cytokine that plays a critical role in the host response to infection and injury.
This antibody can be used together with HEK-Blue™ IL-1β Cells treated with recombinant human IL-1β for screening and neutralization assays to block interleukin-1 signaling.
Key features
- Each lot is functionally tested and validated.
- The complete sequence of the antibody construct has been verified.
The absence of endotoxins is determined by the EndotoxDetect™ assay.
The hybridoma-derived Anti-hIL-1β-IgG (mabg-hil1b-3) antibody has been replaced by Anti-hIL-1β-mIgG1 (mab9-hil1b), which is produced by recombinant technology and purified from CHO cells.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
IL-1β
Human
Sodium phosphate buffer with glycine, saccharose, and stabilizing agents
0.22 µm filtration
Negative (tested using EndotoxDetect™ assay)
Neutralization assay
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Anti-hIL-1β-mIgG1
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Cat code:mab9-hil1b
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Quantity:100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Interleukin-1 beta (IL-1β) is a secreted pro-inflammatory cytokine [1]. It participates in the generation of systemic and local responses to infection and injury [2]. IL-1β is produced by activated macrophages as a pro‑protein, which is cleaved by caspase 1, an enzyme that is activated within the inflammasome multiprotein complex [3].
The resulting mature IL-1β is secreted and binds to the IL-1RI receptor triggering the formation of the IL-1R1/IL-1R3/MyD88 complex and inducing MyD88‑mediated intracellular signaling. This leads to the activation of the transcription factor NK-κB signaling, and the JNK and p38 mitogen‑activated protein kinase pathways, which induce the expression of inflammatory cytokines and chemokines, such as IL-6 and IL-8 [4].
1. Dinarello C., 2018. Overview of the IL-1 family in innate inflammation and acquired immunity. Immunol Rev. 281(1): 8-27.
2. Sims J. & Smith D., 2010. The IL-1 family: regulators of immunity. Nat Rev Immunol. 10(2):89-102.
3. O’Neill L., 2008. The interleukin-1 receptor/Toll-like receptor superfamily: 10 years of progress. Immunol. Rev. 226:10–18.
4. Weber A. et al., 2010. Interleukin-1 (IL-1) pathway. Sci Signal. 3(105):cm1.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
No ressource available.
Certificate of analysis
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