Recombinant human IL-10 protein - Bioactive cytokine

Human IL-10 protein - Mammalian cell-expressed, tag-free, carrier-free

Recombinant human IL-10 is a high-quality and biologically active cytokine, validated using proprietary IL-10 reporter cells. This founding member of the IL-10 cytokine family is produced in HEK cells to ensure protein glycosylation and bona fide 3D structure.

Recombinant human IL-10 can be used together with HEK-Blue™ IL-10 cells for the screening of inhibitory molecules, such as therapeutic monoclonal antibodies (see figures).

IL-10 signaling and biological functions

Key features

• Each lot is validated using HEK-Blue™ IL-10 cells
• Endotoxin ≤ 0.01 EU/µg
• 0.2 µm sterile-filtered

Applications

• Standard for IL-10 detection and quantification
• Screening and release assays for antibodies blocking IL-10 signaling
• Screening and release assays for engineered IL-10

Interleukin 10 (IL-10) is an anti-inflammatory cytokine with a crucial role in preventing inflammatory and autoimmune pathologies. While it is known for its immunosuppressive role in conditions like inflammatory bowel disease and chronic infections, it also shows a surprising protective effect in cancer.

 More details

All InvivoGen products are for internal research use only and not for human or veterinary use.

Figures

SDS PAGE of the recombinant human (h)IL-10 protein. 2 μg of hIL-10 was loaded on a 12% Mini-PROTEAN® TGX Stain-Free™ Precast Gel (Bio-Rad), stained with Coomassie. Detection was performed as per the manufacturer’s instructions. A band was detected at ~18 kDa.

Dose-response of HEK-Blue™ IL-10 cells to recombinant IL-10. Cells were stimulated with increasing concentrations of recombinant human IL-10 (hIL-10). After overnight incubation, the STAT3-induced SEAP activity was determined using QUANTI-Blue™, a SEAP detection reagent. Data are shown as optical density (OD) at 650 nm (mean ± SEM).

Specifications

Synonym: Cytokine synthesis inhibitory factor, CSIF, IL10A, MGC126450, MGC126451, TGIF

Source: Human embryonic kidney (HEK) 293 cells

Species: Human

Carrier: Carrier-free

Tag: Tag-free

Accession number: P22301

Protein size (predicted): 160 a.a.  (S19-N178)

Molecular weight: ~ 18 kDa (SDS-PAGE)

Solubility: 100 μg/ml in water

Formulation: Phosphate buffer saline (pH 7.4), 5% saccharose

Form:  Lyophilized

Reconstitution buffer:  Endotoxin-free water (provided)

Purity:  ≥95% (SDS-PAGE)

Endotoxin: The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK‑Blue™ TLR4 cells.

Tested applications:  Cellular assays

Quality control: Each lot is functionally tested and validated.

Contents

Recombinant human IL-10 is provided lyophilized and is available in two quantities:

• rcyc-hil10: 10 µg
• rcyc-hil10-5: 5 x 10 µg
• 1.5 ml endotoxin-free water for rcyc-hil10 and rcyc-hil10-5

 Recombinant hIL-10 is shipped at room temperature.

 Upon receipt, the product should be stored at -20°C.

 Avoid repeated freeze-thaw cycles.

Details

Interleukin 10 (IL-10) is the founding member of the IL-10 cytokine family, which comprises IL-10, IL-19, IL-20, IL-22, IL-24, IL-26, IL-28A (IFN-λ2), IL-28B (IFN-λ3), and IL-29 (IFN-λ1)1. IL-10 was originally depicted as a key mediator of anti-inflammatory responses. While the immunosuppressive action of IL-10 is illustrated by its genetic association with inflammatory bowel disease or its upregulated expression in chronic infections, an unanticipated IL-10 protective role has been reported in cancer [1].
IL-10 also plays important homeostatic roles in non-immune cells, including neurons, adipocytes, and epithelial cells [1]. The manipulation of IL-10 production and signaling has thus become an attractive therapeutic strategy [2].
Upon IL-10 binding, the IL-10Rα chain oligomerizes with the IL-10Rβ chain. This, in turn, allows the phosphorylation of JAK1 (Janus kinase 1) and TyK2 (Tyrosine kinase 2), and the subsequent activation of STAT3 (Signal transducer and activator of transcription 3) [1]. Importantly, STAT3 is also activated downstream of other cytokine receptors. How the cells trigger distinct and specific gene expression in response to cytokine signaling through the same transcription factor is not fully understood yet [1].

1. Saraiva M, et al., 2019. Biology and therapeutic potential of interleukin-10. J.  Exp. Med. doi: 10.1084/jem.20190418.
2. Ouyang W & O’Garra A, 2019. IL-10 family cytokines IL-10 and IL-22: from basic science to clinical translation. Immunity. 50:871-91.