Human IFN-β Antibody

Human IgG1 (clone 10B10) - Recombinant

ABOUT

Recombinant anti-human interferon-beta neutralizing monoclonal antibody

Anti-hIFN-β-hIgG1 is a monoclonal antibody (mAb) targeting human interferon-beta (hIFN-β).

It was previously extracted from hybridoma cells (clone 10B10). It is now expressed and produced in Chinese hamster ovary (CHO) cells, ensuring reliability and lot-to-lot reproducibility. This antibody has been selected for its ability to efficiently neutralize the biological activity of hIFN-β.

IFN-β is an important anti-viral cytokine that also has anti-proliferative and immunomodulatory functions [1, 2].

More details More details

 

This antibody can be used together with HEK-Blue™ IFN-α/β Cells treated with recombinant human IFN-β for screening and neutralization assays to study IFN-β-induced signaling (see figure).

 

Key features

  • Each lot is functionally tested and validated.
  • The complete sequence of the antibody construct has been verified.
  • The absence of endotoxins is determined by the EndotoxDetect™ assay.

 

The hybridoma-derived Anti-hIFN-β-IgG (mabg2-hifnb-3) antibody has been replaced by Anti-hIFN-β-hIgG1 (mab1-hifnb), which is produced by recombinant technology and purified from CHO cells. It now features a human IgG1 constant region.

References:

1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.

All InvivoGen products are for internal research use only, and not for human or veterinary use.

Binding of Anti-hIFN-β mAb
Binding of Anti-hIFN-β mAb

InvivoGen also offers:

SPECIFICATIONS

Specifications

Target

IFN-β

Target species

Human

Applications

Neutralization (tested)

ELISA

Antibody applications
Neutralization assay
Species
Human
Isotype
hIgG1
kappa
Recommended isotype control
Human IgG1 Isotype Control Antibody
Clone
10B10
Tag
Tag-free
Source
CHO cells
Production details
Animal-free
Purification
Protein G
Formulation buffer

Sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Purity
≥ 95 %
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Neutralization assay

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hIFN-β-hIgG1
  • Cat code: 
    mab1-hifnb
  • Quantity: 
    100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20 °C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Type I interferons, in particular interferon-alpha (IFN-α) and interferon beta (IFN-β), play a vital role in host resistance to viral infections [1, 2]. The type I IFN family is a multi-gene cytokine family that encodes 13 partially homologous IFN-α subtypes in humans (14 in mice), a single IFN-β, and several poorly defined single gene products (IFN-ɛ, IFN-τ, IFN-κ, IFN-ω, IFN-δ, and IFN-ζ) [1, 2].  IFN-α and IFN-β are the best-defined and most broadly expressed type I IFNs [2].

IFN-β and all of the IFN-α subtypes bind to a heterodimeric transmembrane receptor composed of the subunits IFNAR1 and IFNAR2 which are associated with the tyrosine kinases Tyk2 and Jak1 (Janus kinase 1) respectively. These kinases phosphorylate STAT1 and STAT2 which then dimerize and interact with IFN regulatory factor 9 (IRF9), leading to the formation of the ISGF3 complex. ISGF3 binds to IFN-stimulated response elements (ISRE) in the promoters of IFN-stimulated genes (ISG) to regulate their expression. 

 

1. Schreiber G. 2017. The molecular basis for differential type I interferon signaling. J. Biol. Chem. 292:7285-94.
2. McNab F. et al., 2015. Type I interferons in infectious disease. Nat Rev Immunol. 15(2):87-103.

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