Anti-hTLR1-IgG

Human TLR1 Neutralizing antibody - Monoclonal Mouse IgG1 (H2G2)

Anti-hTLR1-IgG (H2G2) is a monoclonal IgG isotype 1 antibody specific for human Toll-like receptor 1 (hTLR1, CD281).

Specifications

Clonality: Monoclonal antibody

Applications: Neutralizing human TLR1-induced cellular activation

Specificity: Human TLR1

Clone: H2G2

Isotype: Mouse IgG1

Formulation: phosphate buffered saline (PBS; pH 7.4) with 5% saccharose

Contents

• 100 μg purified monoclonal anti-hTLR1 IgG antibody (anti-hTLR1-IgG) provided lyophilized.

Product is shipped at room temperature.

Store lyophilized anti-hTLR1-IgG at -20°C.

Lyophilized anti-hTLR1-IgG is stable for 1 year at -20°C. Resuspended anti-hTLR1-IgG is stable up to 3 months when stored at -20°C.

Description

Immunity to invading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 (aka TLR11) and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR1 is predominantly expressed in the spleen and peripheral blood cells. No direct ligands have been identified so far for TLR1, and its function remains unclear. TLR1 seems to act as a coreceptor for TLR2. TLR1 and TLR2 form heterodimeric complexes on the cell surface and in the cytosol [1]. TLR1 and TLR2 were shown to cooperate in recognizing Borrelia burgdorferi outer-surface protein A lipoprotein OspA [2]. They also interact to recognize the 19-kD mycobacterial lipopeptide and several synthetic triacylated lipopeptides [3], but not diacylated lipopeptides. This suggests that TLR1 is able to discriminate among lipoproteins by recognizing the lipid configuration [4].

1. Sandor F. et al., 2003. Importance of extra- and intracellular domains of TLR1 and TLR2 in NFkappa B signaling. J Cell Biol. 2003 Sep 15;162(6):1099-110.
2. Alexopoulou L. et al., 2002. Hyporesponsiveness to vaccination with Borrelia burgdorferi OspA in humans and in TLR1- and TLR2-deficient mice. Nat Med. 8(8):878-84.
3. Takeuchi O. et al., 2002. Cutting edge: role of toll-like receptor 1 in mediating immune response to microbial lipoproteins. J Immunol, 169(1):10-4.
4. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol, 13(7):933-40.