Anti-hTLR6-IgG

Human TLR6 Neutralizing antibody - Monoclonal Mouse IgG1 (C5C8)

Anti-hTLR6-IgG (C5C8) is a monoclonal antibody specific for human Toll-like receptor 6 (hTLR6, CD286).

TLR6, which is closely related to TLR1, is expressed predominantly in spleen, thymus, ovary, and lung [1]. This receptor co-operates with TLR2 to recognize diacylated lipoproteins (LPs) found in mycoplasma but is not required in the recognition of triacylated LPs found in bacteria [2].

This suggests that TLR6 is able to discriminate between the N- terminal lipoylated structures of LPs derived from mycoplasma and bacteria. TLR6 collaboration with TLR2 is essential for the recognition and signal transduction of diacylated LPs, such as MALP- 2 and FSL-1 [2, 3], as well as heat-killed mycoplasma, indicating that TLR6 and TLR2 play a central role in recognizing mycoplasma [2].

Anti-hTLR6-IgG can be used for neutralization of hTLR6, it blocks cellular activation induced by agonists that are recognized by TLR6 and TLR2, such as FSL-1.

References

1. Takeuchi O. et al., 1999. TLR6: A novel member of an expanding toll-like receptor family. Gene. 231(1-2):59-65.
2. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol, 13(7):933-40.
3. Fujita M. et al., 2003. Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. J Immunol. 171(7):3675-83.

Specifications

Clonality: Monoclonal antibody

Applications: Neutralization of human TLR6-induced cellular activation

Specificity: Human TLR6

Clone: C5C8

Isotype: Mouse IgG1, kappa

Formulation: 0.2 µm filtered solution in PBS with 5% saccharose

Contents

• 100 μg purified monoclonal anti-hTLR6 IgG antibody (anti-hTLR6-IgG) provided lyophilized.

Product is shipped at room temperature.

Store lyophilized anti-hTLR6-IgG at -20°C.

Lyophilized anti-hTLR6-IgG is stable for 1 year at -20°C. Resuspended anti-hTLR6-IgG is stable up to 3 months when stored at -20°C.

Description

Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 (aka TLR11) and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR6, which is closely related to TLR1, is expressed predominantly in spleen, thymus, ovary and lung [1]. TLR6 cooperates with TLR2 to recognize diacylated lipoproteins (LPs) found in mycoplasma but is not required in the recognition of triacylated LPs found in bacteria [2]. This suggests that TLR6 is able to discriminate between the N-terminal lipoylated structures of LPs derived from mycoplasma and bacteria. TLR6 collaboration with TLR2 is essential for the recognition and signal transduction of diacylated LPs, such as MALP-2 and FSL-1 [2, 3], as well as heat-killed mycoplasma, indicating that TLR6 and TLR2 play a central role in recognizing mycoplasma [2].

1. Takeuchi O. et al., 1999. TLR6: A novel member of an expanding toll-like receptor family. Gene. 231(1-2):59-65.
2. Takeuchi O. et al., 2001. Discrimination of bacterial lipoproteins by Toll-like receptor 6. Int Immunol, 13(7):933-40.
3. Fujita M. et al., 2003. Involvement of leucine residues at positions 107, 112, and 115 in a leucine-rich repeat motif of human Toll-like receptor 2 in the recognition of diacylated lipoproteins and lipopeptides and Staphylococcus aureus peptidoglycans. J Immunol. 171(7):3675-83.