Anti-hCD19-CD3
Anti-hCD19-CD3 | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
Bispecific antibody against human CD19 and human CD3 |
10 µg 50 µg |
bimab-hcd19cd3 |
Anti-hCD19-CD3 binds to hCD3 on T cells and to hCD19 on B cells
Monoclonal scFv antibody against human CD19 and human CD3
Anti-hCD19-CD3 is a bispecific antibody that recognizes two human cell markers: hCD19, which is expressed on the surface of B cells, and hCD3 which is part of the T cell receptor. This antibody features Blinatumomab single-chain variable fragments (scFv) joined by a glycine-serine linker. These two scFvs have been cloned from the anti-hCD19 (clone HD37) and anti-hCD3 (clone L2K-07) monoclonal antibodies [1,2]. Blinatumomab is used for the treatment of refractory acute lymphocytic leukemia (ALL) in clinics. By binding to hCD3 and hCD19, Blinatumomab engages unstimulated T cells to proliferate and exert cytotoxic activity on CD19-positive lymphoma cells [2]. Blinatumomab does not cross‑react with CD3 and CD19 from mice, rats, or dogs [3].
Anti-hCD19-CD3 bispecific antibody can be used for fine-tuning studies of B cell contact-dependent killing and T cell activation/proliferation.
Negative control antibodies are also available from InvivoGen: Anti-hCD19-βGal and Anti-βGal-hCD3.
References:
1. Krishnamurthy A. & Jimeno A., 2017. Bispecific antibodies for cancer therapy: A review. Pharmacol Ther. S0163-7258(17)30293-0.
2. Bargou R. et al., 2008. Tumor Regression in Cancer Patients by Very Low Doses of a T Cell– Engaging Antibody. Science. 321(5891):974-7.
3. Trivedi A. et al., 2017. Clinical Pharmacology and Translational Aspects of Bispecific Antibodies. Clin Transl Sci. 10(3):147-162.
Specifications
Clonality: Monoclonal antibody.
Specificity: Targets human CD19 and human CD3.
Isotype: none (scFv).
Source: CHO (Chinese hamster ovary) cells.
Purity: > 90% Purified by HisTrap affinity chromatography.
Quality control:
- Binding to hCD19 and to hCD3 has been confirmed by flow cytometry.
- Biological activity has been confirmed using cellular assays.
- The complete sequence of this antibody has been verified.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
Contents
10 µg Anti-hCD19-CD3, purified antibody, provided azide-free and lyophilized.
Product is shipped at room temperature.
Store lyophilized antibody at -20°C.
Lyophilized product is stable for at least 1 year.