|Anti-hCD19-CD3||Unit size||Cat. code||Docs||Qty||Price|
Bispecific antibody against human CD19 and human CD3
Monoclonal scFv antibody against human CD19 and human CD3
Anti-hCD19-CD3 is a bispecific antibody that recognizes two human cell markers: hCD19, which is expressed on the surface of (malignant) B cells, and hCD3 which is part of the T cell receptor. This antibody features blinatumomab’s single-chain variable fragments (scFv) joined by a glycine-serine linker and a hexahistidine-tag (His6). Blinatumomab was approved by the FDA as the second line treatment of refractory acute lymphocytic leukemia (ALL) .
- Reacts with human CD19 and human CD3
- ScFv of clinical relevant bispecific mAb blinatumomab
- Hexahistidine (His6) tag
- Provided azide-free
- Each lot is functionally tested
- Adjustment studies of B cell contact-dependent killing
- Improving T cell activation
1. Krishnamurthy A. & Jimeno A., 2017. Bispecific antibodies for cancer therapy: A review. Pharmacol Ther. S0163-7258(17)30293-0.
Jurkat-Lucia™ NFAT cell activation upon incubation with Raji B cells and Anti-hCD19-CD3.
A. Evaluation of T cell activation. Raji cells were pre-incubated with Anti-hCD19-CD3, or the two bispecific negative controls Anti-β-Gal-hCD3 and Anti-hCD19-β-Gal for 30 minutes before addition of Jurkat-Lucia™ NFAT cells. After 8 hours incubation, T cell activation was determined by measuring the Lucia luciferase activity using QUANTI-Luc™ detection reagent. Results are presented as relative activity expressed as a percentage of Lucia activity in samples with the highest dose of antibody.
B. Target specificity of Anti-hCD19-CD3 was verified upon incubation with Jurkat-Lucia™ NFAT cells and Raji cells as described in (A), with Jurkat-Lucia™ NFAT cells only, or with Raji cells only. T cell activation was determined by measuring the Lucia luciferase activity using QUANTI-Luc™ and expressed as relative light units (RLUs).
Clonality: Monoclonal antibody
Clone: HD37 (Anti-hCD19) and L2K-07 (Anti-hCD3)
Specificity: Targets human CD19 and human CD3
Isotype: none (scFv)
Source: CHO (Chinese hamster ovary) cells
Purity: > 90% Purified by HisTrap affinity chromatography
Tested application: FACS, cellular assays
- Binding to hCD19 and to hCD3 has been confirmed by flow cytometry.
- Biological activity has been confirmed using cellular assays.
- The complete sequence of this antibody has been verified.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
10 µg Anti-hCD19-CD3, purified antibody, provided azide-free and lyophilized.
Product is shipped at room temperature.
Store lyophilized antibody at -20°C.
Lyophilized product is stable for at least 1 year.Back to the top
The cluster of differentiation 19 (CD19) is a transmembrane protein acting as a co-receptor for the B cell receptor (BCR) modulating B lymphocyte differentiation and activation . CD19 is expressed throughout the entire B cell maturation process and can be found in most lymphoid malignancies including acute lymphocytic leukemia (ALL), chronic lymphocytic leukemia (CLL) and Non-Hodgkin lymphoma (NHL). Therefore, CD19 is an interesting target for the development of experimental therapeutic agents in immunotherapy research .
In 2014, the first bispecific T-cell engager (BiTE®) blinatumomab was approved by the FDA as the second line treatment of refractory ALL. By simultaneously binding to hCD3 and hCD19, blinatumomab (AMG103) engages unstimulated T cells to proliferate and exert cytotoxic activity on CD19-positive lymphoma cells .
The cluster of differentiation 3 (CD3, formerly named T3) is a multimeric protein complex consisting of four polypeptides (CD3ε, CD3γ, CD3δ, and CD3ζ) that assemble as three dimers (εγ, εδ, and ζζ) . It is a marker of T cells, which recognizes and participates in the elimination of infected cells and tumor cells through the interaction between the TCR (T cell receptor) on T cells and the MHC-peptide complex on antigen-presenting cells [4-5]. Because of its short cytoplasmic tail, the TCR lacks the ability to signal and requires non-covalent association with the CD3. Upon antigen recognition, the TCR/CD3 complex on T cells triggers downstream intracellular signaling and participates in T cell activation .
1. Del Nagro et al., 2005. CD 19 function in central and peripheral B-cell development. Immunol Res 31, 119–131 (2005).
2. Katz, Ben-Zion; Herishanu, Yair, 2014. Therapeutic targeting of CD19 in hematological malignancies: past, present, future and beyond. Leukemia & Lymphoma, 55(5), 999–1006.
3. Zinzani, P.L., Minotti, G., 2022. Anti-CD19 monoclonal antibodies for the treatment of relapsed or refractory B-cell malignancies: a narrative review with focus on diffuse large B-cell lymphoma. J Cancer Res Clin Oncol 148, 177–190.
4. Chetty R. & Gatter K., 1994. CD3: structure, function, and role of immunostaining in clinical practice. J. Pathol. 173(4):303-307.
5. Smith-Garvin J.E. et al., 2009. T Cell Activation. Ann. Rev. Immunol. 27:591-619.3.