Human CD3 x Human CD28 Antibody For T Cell Activation & Expansion

T cell activating and expanding bispecific antibody anti-CD3 & anti-CD28

ABOUT

Bispecific T cell engager for effective T cell activation & expansion

The bispecific antibody (bsAb) CD3-CD28 is designed to activate and expand enriched T cell populations or T cells from peripheral blood mononuclear cells (PBMCs).

Its efficacy in activating and expanding enriched CD4+ T cells in the presence of recombinant human IL-2 has been validated using imaging and flow cytometry (see figures).

Key features

  • Cost-effective: 1 ml (100 µg/ml) activates and expands up to 100 Mio. T cells
  • Simple: no need for magnetic beads, feeder cells, or specialized media
  • Versatility: Compatible with transfection or transduction methods

Applications

  • T cell research
  • Cancer immunotherapy studies
  • Understanding T cell responses to bacterial and viral infections
  • Development of CAR-T cells

 

The bsAb CD3-CD28 is a fusion protein dimer comprising tandem single-chain variable fragments (scFv)2 from two monoclonal antibodies (mAbs). These mAbs were derived from the clones OKT3 and 15E8 targeting the human CD3 and CD28 receptors on the cell surface of human T cells, respectively. It mimics the natural cross-linking of these molecules by APCs in vivo and delivers the necessary signal 1 and signal 2 to the T cells for their activation and expansion. This so-called (scFv)2-(scFv)2-Fc molecule belongs to the family of IgG-like antibodies. It features an IgG1 Fc fragment, which contributes to increased solubility, serum half-life, as well as facilitated purification.

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All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Species
Human
Clone
OKT3 (Anti-CD3 ) and 15E8 (Anti-CD28)
Tag
Tag-free
Source
CHO cells
Production details
animal free
Purification
Affinity chromatography
Formulation buffer

Phosphate buffer solution (pH 7.4), 5% saccharose

Preservative
Azide-free
Purity
≥ 95 %
Tested applications

Activation and expansion of T cells confirmed using cellular assays

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    bsAb CD3-CD28
  • Cat code: 
    bsab-tex-1
  • Quantity: 
    100 µg
Notes:

bsAb CD3-CD28 is provided azide-free and lyophilized

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

T cell activation

Critical to almost all functions of the adaptive immune response is the activation of T cells, such as CD8+ cytotoxic T cells and CD4+ helper T cells. The activation of T cells requires two signals:

  • Signal 1: TCR (CD3) engagement with the MHC:peptide complexes on antigen-presenting cells (APCs) [1] and
  • Signal 2: A co-stimulatory signal delivered notably by the interaction between the T cell CD28 receptor and CD80 (B7.1) or CD86 (B7.2) on APCs [2].

Importantly, both signals are required for T cell proliferation, cytokine production (e.g. IL-2, IFN-γ), and cellular functions.

T cell-based immunotherapy is an effective strategy to treat a wide range of cancers. Such therapies rely on the ex-vivo activation and expansion of naturally occurring tumor-specific T cells that are otherwise only retrieved in small numbers from the patient's blood or tissues. Importantly, the inherent T cell immune‑modulating functions need to be preserved. A promising approach for the activation of T cells is the use of bispecific antibodies. These unique molecules bring together the specificities of two antibodies and can bind simultaneously to two separate and unique antigens or epitopes such as CD3 and CD28.

 

References:

1. Brameshuber, M. et al. 2018. Monomeric TCRs drive T cell antigen recognition. Nat Immunol 19, 487-496.
2. Esensten, J.H. et al. 2016. CD28 Costimulation: From Mechanism to Therapy. Immunity 44, 973-988.

DOCUMENTS

Documents

bsAb CD3-CD28

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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