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Anti-βGal-hCD3 [NEW]

Anti-βGal-hCD3 Unit size Cat. code Docs Qty Price
Bispecific antibody against β-galactosidase and human CD3; Negative control
10 µg
bimab-bgalhcd3
+-
$325.00

NEW PRODUCT

Monoclonal scFv antibody against β-galactosidase and human CD3; Negative control

Anti-βGal-hCD3 is a bispecific antibody that binds to two sites: E. coli β-galactosidase (β-Gal)and hCD3, part of the T cell receptor. It is used as a negative control for Anti-hCD19-CD3 bispecific antibody that, alike Blinatumomab, engages unstimulated T cells to proliferate and exert cytotoxic activity on CD19-positive lymphoma cells [1,2].

 

References:

1. Krishnamurthy A. & Jimeno A., 2017. Bispecific antibodies for cancer therapy: A review. Pharmacol Ther. S0163-7258(17)30293-0.
2. Bargou R. et al., 2008. Tumor Regression in Cancer Patients by Very Low Doses of a T Cell– Engaging Antibody. Science. 321(5891):974-7. 

 

Figures

Anti-βGal-hCD3 binds to hCD3 but not to hCD19
Anti-βGal-hCD3 binds to hCD3 but not to hCD19

Anti-βGal-hCD3 binds to hCD3 on T cells but not to hCD19 on B cells.

Anti-βgal-hCD3 does not allow Jurkat-Lucia™ NFAT cell activation
Anti-βgal-hCD3 does not allow Jurkat-Lucia™ NFAT cell activation

Anti-βgal-hCD3 does not allow Jurkat-Lucia™ NFAT cell activation upon incubation with Raji B cells.

Activation of Jurkat-Lucia™ NFAT cells with Anti-hCD19-CD3
Activation of Jurkat-Lucia™ NFAT cells with Anti-hCD19-CD3

(A) Raji cells were pre-incubated with Anti-hCD19-CD3, or the two bispecific negative controls Anti-β-Gal-hCD3 and Anti-hCD19-β-Gal for 30 minutes before addition of Jurkat-Lucia™ NFAT cells. After 8 hours incubation, T cell activation was determined by measuring the Lucia luciferase activity using QUANTI-Luc™ detection reagent. Results are presented as relative activity expressed as a percentage of Lucia activity in samples with the highest dose of antibody.
(B) Target specificity of Anti-hCD19-CD3 was verified upon incubation with Jurkat-Lucia™ NFAT cells and Raji cells as described in (A), with Jurkat-Lucia™ NFAT cells only, or with Raji cells only. T cell activation was determined by measuring the Lucia luciferase activity using QUANTI-Luc™ and expressed as relative light units (RLUs).

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Specifications

Clonality: Monoclonal antibody.

Specificity: E. coli β-galactosidase (β-Gal) and human CD3.

Isotype: none (scFv).

Source: CHO (Chinese hamster ovary) cells.

Purity: >90%. Purified by affinity chromatography.

Quality control:

  •    Binding to hCD3 has been confirmed by flow cytometry.
  •    The inability of Anti-βGal-hCD3 to induce T cell activation has been confirmed using cellular assays.
  •    The complete sequence of this antibody has been verified.
  •    The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
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Contents

10 µg Anti-βGal-hCD3, purified antibody, provided azide-free and lyophilized.

Product is shipped at room temperature.

Store lyophilized antibody at -20°C.

Lyophilized product is stable for at least 1 year.

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