Human TROP2 Antibody for ADC - Sacituzumab Biosimilar

Human IgG1 - CAS #1796566-95-4 - For antibody-drug conjugation

ABOUT

Monoclonal human IgG1 antibody against TROP2 for antibody-drug conjugation

InvivoGen provides Anti-TROP2-hIgG1, a monoclonal antibody (mAb) specifically developed for the generation of antibody-drug conjugates (ADCs). This mAb features:

  • the variable region of sacituzumab, which specifically targets the human trophoblast cell-surface antigen 2 (TROP2),
  • a human IgG1 constant region that displays high effector functions, including antibody-dependent cell-mediated cytotoxicity (ADCC), and complement-dependent cytotoxicity (CDC).

InvivoGen's Anti-TROP2-hIgG1 has been generated by recombinant DNA technology, produced in CHO cells, and purified by affinity chromatography. The antibody formulation contains no tris, cysteine, glycine, or any other amine or thiol function.

 

TROP2 background

TROP2 (trophoblast cell-surface antigen 2), also known as TACD2 (tumor-associated calcium signal transducer 2), is a transmembrane protein expressed in normal human tissues, including breast, cervix, kidney, lung, and pancreas, and is upregulated in most tumors of epithelial origin [1]. TROP2 plays an important role in embryonic development and is implicated in several oncogenetic signaling pathways leading to proliferation [1].
One therapeutic strategy aiming at killing TROP2+ cancer cells, notably in triple-negative breast cancer patients, uses the FDA-approved antibody-drug conjugate (ADC) Trodelvy [2,3]. It consists of the combination of the Anti-TROP2 sacituzumab and the SN-38 cytotoxic payload [2,3]. This ADC allows targeted drug delivery to cancer cells in addition to the mAb-mediated effector functions [2,3]. Other TROP2-targeting ADCs featuring different cytotoxic cargos have been developed and have progressed to human clinical trials [4].

In a co-culture of TROP2+ tumor cells and human peripheral blood monocytes (PBMCs), ADCs combining InvivoGen's Anti-TROP2-hIgG1 and STING or TLR7 agonists induce a significantly higher production of IL-6 and CXCL10 pro-inflammatory cytokines than unconjugated agonists or negative control ADCs (see Figures).

 

Key features of Anti-TROP2-hIgG1

  • Clinically-relevant variable region targeting human TROP2 (sacituzumab)
  • Human IgG1 constant region for high effector functions
  • Functionally validated by flow cytometry and ADC-based cellular assays
  • The absence of bacterial contamination has been confirmed

 

InvivoGen offers conjugatable PRR ligands for building immunostimulatory Anti-TROP2 ADCs.
You may choose from TLR7 and STING agonists:
Conjugatable TLR7 agonists: TL7-887 and TL7-975
Conjugatable STING agonists: STG-982 and STG-968

 

learn moreLearn more about bioconjugation

 

References

1. Trerotola, M., et al., 2013. Upregulation of Trop-2 quantitatively stimulates human cancer growth. Oncogene 32, 222–233.
2. Drago J.Z. et al., 2021. Unlocking the potential of antibody–drug conjugates for cancer therapy. Nat Rev Clin Oncol. 18-6):327.
3. Tarantino P. et al., 2022. Antibody-drug conjugates: smart chemotherapy delivery across tumor histologies. CA Cancer J Clin. 72:165-182.
4. Shaffer, C. 2021. Trop2 deal heats up antibody–drug conjugate space in cancer. Nat Biotechnol 39, 128–130.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

TROP2

Target species

Human

Species
Human
Isotype
hIgG1
kappa
Clone
Sacituzumab
CAS number
1796566-95-4
Synonyms
Sacituzumab-Govitecan
Trodelvy
Tag
Tag-free
Source
CHO cells
Purification
Protein A
Formulation buffer

Sodium chloride, sodium phosphate buffer, saccharose

Preservative
Azide-free
Appearance (form)
Lyophilized
Reconstitution buffer
Sterile water (not provided)
Endotoxin

Negative (tested using EndotoxDetect™ assay)

Tested applications

Flow cytometry, ELISA, Antibody-drug conjugation

Quality control

Each lot is functionally tested and validated.

Additional information

 The antibody formulation contains no tris, cysteine, glycine, or any other amine or thiol function.

CONTENTS

Contents

  • Product: 
    Anti-TROP2-hIgG1
  • Cat code: 
    trop2-mab1-1
  • Quantity: 
    1 mg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20 °C
    Stability: -20°C for up to 1 year

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

Anti-TROP2-hIgG1

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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