HEK-Blue™ hTLR2 Cells
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Cat.code:
hkb-htlr2
- Documents
ABOUT
NF-κB–SEAP reporter HEK293 cells expressing human TLR2
HEK-Blue™-hTLR2 cells were obtained by co-transfection of the human TLR2 and SEAP (secreted embryonic alkaline phosphatase) genes into HEK293 cells. The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induce the production of SEAP. Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ hTLR2 cells is HEK-Blue™ Null1 cells.
Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.
SPECIFICATIONS
Specifications
TLR2
Human
TLR2 activation cellular assays
Complete DMEM (see TDS)
Verified using Plasmotest™
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:HEK-Blue™ hTLR2 Cells
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Cat code:hkb-htlr2
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Quantity:3-7 x 10^6 cells
- 2 x 1 ml HEK-Blue™ Selection (250 X)
- 1 ml Normocin® (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium & SEAP detection reagent)
Shipping & Storage
- Shipping method: Dry ice
- Liquid nitrogen vapor
Storage:
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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