Invivogen
Menu

Human TLR2 Reporter HEK293 Cells (NF-κB)

Product Unit size Cat. code Docs. Qty. Price

HEK-Blue™ hTLR2 cells

Human TLR2 expressing HEK293 reporter cells (NF-κB pathway)

Show product

3-7 x 10e6 cells

hkb-htlr2
+-
$1,457

NF-κB–SEAP reporter HEK293 cells expressing human TLR2

HEK-Blue™-hTLR2 cells were obtained by co-transfection of the human TLR2 and SEAP (secreted embryonic alkaline phosphatase) genes into HEK293 cells.

The SEAP reporter gene is placed under the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites.

Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response.

Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induce the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ hTLR2 cells is HEK-Blue™ Null1 cells.

 

Read our review on TLR2

 

Figures

Response of HEK-Blue™ hTLR2 cells to TLR and NOD agonists
Response of HEK-Blue™ hTLR2 cells to TLR and NOD agonists

HEK-Blue™ hTLR2 cells were stimulated with various TLR and NOD  agonists: Pam3CSK4 (100 ng/ml), Poly(I:C) (50 ng/ml), LPS-EB ultrapure (100 ng/ml), recombinant flagellin from S. typhimurium (10 ng/ml), CL264 (1 µg/ml), CL097 (3 μg/ml), ssRNA40/LyoVec™ (5 μg/ml), ODN 2006 (10 μg/ml), C12-iE-DAP (100 ng/ml), L18-MDP (100 ng/ml), and TNF-α (100 ng/ml). After 18h incubation (24h incubation for CL264, C12-iE-DAP and L18-MDP ligands), NF-κB-induced SEAP activity was assessed using QUANTI-Blue™ and by reading the OD at 655 nm.

Response of HEK-Blue™ hTLR2 to TLR2 agonists
Response of HEK-Blue™ hTLR2 to TLR2 agonists

HEK-Blue™ hTLR2 and HEK-Blue™ Null1 (control) cells were incubated in HEK-Blue™ Detection medium and stimulated with 0.1 ng/ml FSL-1 (TLR2/6), 10 ng/ml Pam3CSK4 (TLR1/2), 107 cells/ml HKLM, 1 µg/ml LTA-SA, 10 ng/ml LPS-PG, or 1 µg/ml PGN-BS. After 24h incubation, the levels of NF-κB-induced SEAP were determined by reading the OD at 655 nm.

Neutralization of TLR1/2 dose response
Neutralization of TLR1/2 dose response

Anti-hTLR1 IgG         IC50 400 +/-100 ng/ml
Anti-hTLR2 IgA2       IC50 350 +/-50 ng/ml

HEK-Blue™ TLR2 cells were pre-incubated for 1h with increasing concentrations of anti-hTLR2-IgA, anti-hTLR6-IgG or anti-hTLR1-IgG then stimulated with 0.5 ng/ml of Pam3CSK4 for 18h. NF-kB-induced SEAP activity was assessed using QUANTI-Blue™ by reading the OD at 655 nm.

Neutralization of TLR2/6 dose response
Neutralization of TLR2/6 dose response

Anti-hTLR2 IgA2       IC50 300 +/-100 ng/ml
Anti-hTLR6 IgG         IC50 60 +/-20 ng/ml

HEK-Blue™ TLR2 cells were pre-incubated for 1h with increasing concentrations of anti-hTLR2-IgA, anti-hTLR6-IgG or anti-hTLR1-IgG then stimulated with 0.1 ng/ml of FSL-1 for 18h. NF-kB-induced SEAP activity was assessed using QUANTI-Blue™ by reading the OD at 655 nm.

Back to the top

Specifications

Antibiotic resistance: blasticidin, hygromycin, Zeocin®

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™

Guaranteed mycoplasma-free.

 

This product is covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

Dry ice shipping Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Back to the top

FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

Back to the top

Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

Customer Service
& Technical Support
Shopping cart is empty