Anti-hTLR2-IgA
| Anti-hTLR2-IgA | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Human TLR2 Detection and Neutralizing antibody - Monoclonal Human IgA2 (B4H2) |
100 µg |
maba2-htlr2 |
Anti-hTLR2-IgA is a chimeric monoclonal antibody specific for human Toll-like receptor 2 (TLR2, CD282). It was generated by combining the constant domains of the human IgA molecule with murine variable regions. This antibody has been selected for its ability to efficiently neutralize the biological activity of TLR2. The neutralizing activity of this IgA antibody was determined using HEK-Blue™ TLR2 Cells. It was found to be significantly higher than the neutralizing activity of the corresponding IgG isotype.
Specifications
Clonality: Monoclonal antibody
Application: Neutralization of human TLR2-induced cellular activation. It can also be used for flow cytometry.
Specificity: Human TLR2
Clone: B4H2
Isotype: Human IgA2
Formulation: 0.2 μm filtered solution in Tris HCl buffer with saccharose, glycine and stabilizing agents
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- 100 μg purified anti-hTLR2-IgA antibody, provided azide-free and lyophilized
Product is shipped at room temperature.
Store lyophilized antibody at -20 °C.
Lyophilized product is stable for at least 1 year.
Description
Toll-Like receptors (TLRs) play a critical role in early innate immunity toinvading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 and TLR13 in mice, the homolog of TLR10 being a pseudogene.
TLR2 is involved in the recognition of a wide array of microbial molecules. TLR2 recognizes lipoteichoic acid and lipoprotein from gram- positive bacteria, lipoarabinomannan from mycobacteria, and zymosan from yeast cell wall. Moreover, TLR2 participates in the recognition of some types of LPS. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of microbial molecules that TLR2 can recognize. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipopeptide [1], and associates with TLR1 to recognize triacylated lipopetides [2]. Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14 [3].
1. Girard R et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116(Pt 2):293-302.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. Proc Natl Acad Sci USA. 97(25):13766-71.
3. Lotz S. et al., 2004. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 75(3):467-77.