Anti-β-Gal-IgA2 isotype controls

Monoclonal human IgA2 antibodies against E. coli β-galactosidase

InvivoGen offers two human monoclonal antibodies (mAbs) targeting E. coli β-galactosidase (β-Gal):

• Anti-β-Gal-hIgA2
• Human IgA2 Control, a mono-glycosylated anti-β -Gal-hIgA2 alternative.

These antibodies are provided as isotype controls for InvivoGen’s IgA2 mAb collection (see below).  

Both mAbs features the constant region of the human IgA2 isotype and the variable region of Mouse IgG2a Control (clone: T9C6) targeting E. coli β-Gal. Therefore, they do not react with human targets.

They were generated by DNA immunization with different plasmids expressing the β-Gal gene in Swiss mice using recombinant technology, produced in Chinese ovary hamster (CHO) cells, and purified by affinity chromatography.

Specifications

Specificity: E. coli β-galactosidase

Clonality: Monoclonal

Clone: T9C6

Isotype: Human IgA2, kappa

Source: CHO cells

Formulation: 0.2 μm filtered solution in Tris/glycine with 5% saccharose

Purity: Affinity chromatography purified

Application: Negative control for the following human IgA2 antibodies (see below)

Quality control:

• The absence of binding of Human IgA2 Control and Anti-β-Gal-hIgA2 to human cell lines has been controlled using flow cytometry.
• The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.

Please use Anti-β-Gal-hIgA2 for the following antibodies:

• Anti-hCD20-hIgA2
• Anti-hTNF-α-hIgA2
• HER2-Tra-hIgA2
• Anti-hRANKL-hIgA2
• Anti-hCTLA4-hIgA2
• Anti-hPD1-Ni-hIgA2
• Anti-hPD1-Pem-hIgA2
• Anti-hCD20-Ga-hIgA2
• Anti-hCD20-Of-hIgA2
• Anti-hEGFR-hIgA2
• Anti-hVEGF-hIgA2

Please use the mono-glycosylated Human IgA2 Control for the following antibodies:

• Anti-hIFNγ-hIgA2
• Anti-hTLR2-IgA
• Anti-hTLR5-IgA2

Contents

Please note: Each mAb is sold separately. See TDS for the exact contents.

• 200 μg purified Anti-β-Gal-IgA2 antibody, provided azide-free and lyophilized

or

• 100 μg purified Control-IgA2 antibody, provided azide-free and lyophilized.

Product is shipped at room temperature.

Store lyophilized antibody at -20°C.

Product is stable for 6 month (Human IgA2 Control) or 1 year (Anti-β-Gal-hIgA2).

Details

The human IgA2 is found predominantly in secretory lymphoid tissues, where it plays a critical role in mucosal immunity. It is highly resistant to enzymatic degradation by bacterial proteases, due to a short hinge region. IgA2 displays no complement-dependent cytotoxicity (CDC) and low antibody-dependent cell-mediated cytotoxicity (ADCC).