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Anti-β-Gal-hIgG1 (N298A)

Anti-β-Gal-hIgG1 (N298A) Unit size Cat. code Docs Qty Price
β-galactosidase antibody - Human IgG1 (N298A)
200 µg
bgal-mab12
+-
$301.00

Monoclonal human IgG1 (N298A) antibody against E. coli β-galactosidase

Anti-β-Gal-hIgG1 (N298A) features the constant region of the human IgG1 (N298A) isotype and the variable region of Mouse IgG2a Control. Mouse IgG2a Control is a mouse IgG2a monoclonal antibody that targets E. coli β-galactosidase (β-Gal).

This antibody was generated by DNA immunization with a plasmid expressing the β-Gal gene in Swiss mice.

Anti-β-Gal-hIgG1 (N298A) can be used as an isotype control for human IgG1 antibodies containing the N298A mutation. Antibodies with this mutation contain a modified Fc region designed to limit antibody-dependent cytotoxicity (ADCC) and complement-dependent cytotoxicity (CDC) [1, 2], as this mutation eliminates their ability to bind to human Fcγ receptors.

Anti-β-Gal-hIgG1 (N298A) was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity  chromatography with protein G.

 

References:

1. Spigel D. et al., 2013. Clinical activity, safety, and biomarkers of MPDL3280A, an engineered PD-L1 antibody in patients with locally advanced or metastatic non-small cell lung cancer (NSCLC) [ASCO abstract 8008]. J Clin Oncol. 31(15)(suppl).
2. Herbst RS. et al., 2014. Predictive correlates of response to the anti-PD-L1 antibody MPDL3280A in cancer patients. Nature. 515(7528):563-7.

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Specifications

Clonality: Monoclonal antibody

Specificity: E. coli β-galactosidase (β-Gal)

Isotype: Human IgG1 (N298A)

Application: Negative control for human IgG1 antibodies

Source: CHO cells

Purity: Purified by affinity chromatography with protein G

Quality control:

  • The absence of binding of Anti-β-Gal-hIgG1 (N298A) to human cell lines has been tested using flow cytometry.
  • The absence of endotoxins and other bacterial contaminants has been confirmed using HEK-Blue™ TLR4 and HEK-Blue™ TLR2 cells.
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Contents

  • 200 µg purified anti-β-Gal-hIgG1 (N298A) antibody, provided azide-free and lyophilized.

room temperature Product is shipped at room temperature.

store Store lyophilized antibody at -20°C.

stability Product is stable for 1 year.

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