Human TLR5 Antibody

Human IgA2 (clone Q2G4) - Recombinant

ABOUT

Human TLR5 Detection and Neutralizing Antibody - Monoclonal Human IgA2

Anti-hTLR5-IgA (clone Q2G4) is a chimeric monoclonal antibody specific for human Toll-like receptor 5 (hTLR5). This antibody was generated by combining the constant domains of the human IgA molecule with the murine variable regions. It was produced in Chinese hamster ovary (CHO) cells and purified by affinity chromatography.

Anti-hTLR5-IgA has been selected for its ability to efficiently neutralize the biological activity of hTLR5. The neutralizing activity was determined using HEK-Blue™ TLR5 cells. This antibody can also be used to detect hTLR5 using flow cytometry. TLR5 recognizes flagellin from Gram-positive and Gram-negative bacteria. Activation of the receptor stimulates the production of proinflammatory cytokines through signaling via the adaptor protein MyD88.

More details More details

 

Key features:

  • Reacts with human TLR5
  • Provided azide-free
  • Each lot is functionally tested

 

Read our review Read our review on Toll-Like Receptors.

 

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Target

Human TLR5 (hTLR5)

Target species

Human

Applications

Neutralizing hTLR5-induced cellular activation, Detection using flow cytometry

Isotype
hIgA2
kappa
Clone
Q2G4
Source
CHO cells
Production details
Animal-free
Purification
Affinity chromatography
Formulation buffer

TRIS with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Reconstitution buffer
Sterile water
Sterility

0.2 µm filtration

Tested applications

Neutralizing hTLR5-induced cellular activation, Detection using flow cytometry

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-hTLR5-IgA2
  • Cat code: 
    htlr5-mab7-2
  • Quantity: 
    2 x 100 µg

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Stable for 1 month when stored at 4°C and for 1 year when aliquoted and stored at -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionarily conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12, and TLR13 in mice, the homolog of TLR10 being a pseudogene.

TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria. Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1-3]. TLR5 can generate a pro-inflammatory signal as a homodimer suggesting that it might be the only TLR participating in flagellin recognition [3]. However, TLR5 may require the presence of a co-receptor or adaptor molecule for efficient ligand recognition and/or signaling [4].

 

1. Yang J. & Yan H. 2017. TLR5: beyond the recognition of flagellin.Cell Mol Immunol. 14(12):1017-1019.
2. Gewirtz A.T. et al., 2001. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol, 167(4):1882-5.
3. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature, 410(6832):1099-103.
4. Tallant T. et al., 2004. Flagellin acting via TLR5 is the major activator of key signaling pathways leading to NF-kappa B and proinflammatory gene program activation in intestinal epithelial cells. BMC Microbiol. 4(1):33.

DOCUMENTS

Documents

Anti-hTLR5-IgA2

Technical Data Sheet

Validation Data Sheet

Safety Data Sheet

Certificate of analysis

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