Anti-mTLR5-IgG
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Cat.code:
mabg-mtlr5-2
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ABOUT
Murine TLR5 Neutralizing antibody - Monoclonal Rat IgG2a
Anti-mTLR5-IgG (clone Q23D11) is a monoclonal antibody specific for murine Toll-like receptor 5 (mTLR5). This monoclonal antibody was produced in hybridoma cells and purified by affinity chromatography.
Anti-mTLR5-IgG has been selected for its ability to efficiently neutralize the biological activity of mTLR5. The neutralizing activity was determined using HEK-Blue™ TLR5 cells. TLR5 recognizes flagellin from Gram-positive and Gram-negative bacteria. Activation of the receptor stimulates the production of proinflammatory cytokines through signaling via the adaptor protein MyD88.
Key features:
- Reacts with murine TLR5
- Provided azide-free
- Each lot is functionally tested
Read our review on Toll-Like Receptors.
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
mTLR5
Neutralization of murine TLR5-induced cellular activation
0.2 µm filtered solution in a sodium phosphate buffer with saccharose, glycine, and stabilizing agents
Neutralization using cellular assays
This product has been validated for neutralization. The absence of bacterial contamination (e.g. lipoproteins and endotoxins) is confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
CONTENTS
Contents
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Product:Anti-mTLR5-IgG
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Cat code:mabg-mtlr5-2
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Quantity:2 x 100 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Toll-like receptors (TLRs) play a critical role in early innate immunity to invading pathogens by sensing microorganisms. These evolutionarily conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12, and TLR13 in mice, the homolog of TLR10 being a pseudogene.
TLR5 recognizes flagellin from both Gram-positive and Gram-negative bacteria. Activation of the receptor stimulates the production of proinflammatory cytokines, such as TNF-α, through signaling via the adaptor protein MyD88 and the serine kinase IRAK [1-3]. TLR5 can generate a pro-inflammatory signal as a homodimer suggesting that it might be the only TLR participating in flagellin recognition [3]. However, TLR5 may require the presence of a co-receptor or adaptor molecule for efficient ligand recognition and/or signaling [4].
1. Yang J. & Yan H. 2017. TLR5: beyond the recognition of flagellin.Cell Mol Immunol. 14(12):1017-1019.
2. Gewirtz AT. et al., 2001. Cutting edge: bacterial flagellin activates basolaterally expressed TLR5 to induce epithelial proinflammatory gene expression. J Immunol. 167(4):1882-5.
3. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
4. Tallant T. et al., 2004. Flagellin acting via TLR5 is the major activator of key signaling pathways leading to NF-kappa B and proinflammatory gene program activation in intestinal epithelial cells. BMC Microbiol. 4(1):33.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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