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Mouse TLR5 Reporter HEK293 Cells (NF-κB)

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HEK-Blue™ mTLR5 cells

Murine TLR5 expressing HEK293 reporter cells (NF-κB pathway)

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3-7 x 10e6 cells

hkb-mtlr5
+-
$1,457

NF-κB–SEAP reporter HEK293 cells expressing mouse TLR5

HEK-Blue™-mTLR5 cells were obtained by co-transfection of the murine TLR5 gene and an inducible SEAP (secreted embryonic alkaline phosphatase) reporter gene into HEK293 cells.

The SEAP gene is placed under the control of the control of the IFN-β minimal promoter fused to five NF-κB and AP-1-binding sites. Stimulation with a TLR5 ligand activates NF-κB and AP-1 which induce the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ mTLR5 cells is HEK-Blue™-Null1-v cells.

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Specifications

Antibiotic resistance: blasticidin, Zeocin®.

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.

Guaranteed mycoplasma-free.

 

This product is covered by a Limited Use License (See Terms and Conditions).

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Contents

Shipped on dry ice Shipped on dry ice (Europe, USA, Canada, and some areas in Asia)

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FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

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Citations

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Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

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