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Rec FLA-ST

Rec FLA-ST Unit size Cat. code Docs Qty Price
Recombinant Flagellin from S. typhimurium
50 µg
10 µg
tlrl-flic-50
+-
$617.00

Recombinant flagellin from S. typhimurium

RecFLA-ST is a recombinant flagellin protein encoded by the fliC gene from the Gram-negative bacteria Salmonella typhimurium.

This protein is produced in CHO cells and purified by affinity chromatography. 

RecFLA-ST is intended for use in cell culture applications. 

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Specifications

Specificity: TLR5 agonist

Working concentration: 10-100 ng/ml

Solubility: 0.5 mg/ml in water

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Contents

Recombinant Flagellin from Salmonella typhimurium (recFLA-ST) is provided lyophilized and is available in two quantities:

tlrl-flic-10:
- 10 μg Rec FLA-ST
- 1.5 ml sterile endotoxin-free water

tlrl-flic-50:
- 50 μg Rec FLA-ST
- 10 ml sterile endotoxin-free water

Recombinant flagellin is shipped at room temperature and should be stored at -20°C.
Upon resuspension, prepare aliquots of recFLA-ST and store at -20°C.

Resuspended product is stable 6 months at -20°C when properly stored. Avoid repeated freeze-thaw cycles.

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Description

Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species. This proinflammatory molecule is present in both Gram-negative and Gram-positive bacteria. It is recognized by distinct types of pattern recognition receptors (PRRs).
Extracellular flagellin is recognized by surface localized Toll-like receptor 5 (TLR5), which promotes the activation of NF-κB and the subsequent production of cytokines [1, 2]. Flagellin is also recognized by the cytosolic NOD-like receptors (NLRs) NAIP5/NAIP6 sensors of the NLRC4 inflammasome [3, 4]. Flagellin monomers are translocated into the host cell cytosol by a mechanism that requires bacterial secretion systems. This triggers the formation of a NAIP-NLRC4 inflammasome leading to caspase-1-mediated secretion of IL-1β and IL-18 [3, 4]. 

1. Song WS. et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Sci Rep. 7:40878.
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
3. Duncan JA. & Canna SW., 2018. The NLRC4 Inflammasome. Immunol Rev. 281(1):115-123.
4. Zhao Y. et al., 2011. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 477(7366):596-600.

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Citations

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