Rec FLA-ST
| Rec FLA-ST | Unit size | Cat. code | Docs | Qty | Price |
|---|---|---|---|---|---|
Recombinant Flagellin from S. typhimurium |
50 µg 10 µg |
tlrl-flic-50 |
Recombinant flagellin from S. typhimurium
RecFLA-ST is a recombinant flagellin protein encoded by the fliC gene from the Gram-negative bacteria Salmonella typhimurium.
This protein is produced in CHO cells and purified by affinity chromatography.
RecFLA-ST is intended for use in cell culture applications.
Back to the topSpecifications
Specificity: TLR5 agonist
Working concentration: 10-100 ng/ml
Solubility: 0.5 mg/ml in water
Back to the topContents
Recombinant Flagellin from Salmonella typhimurium (recFLA-ST) is provided lyophilized and is available in two quantities:
Catalog code: tlrl-flic-10
- 10 μg Rec FLA-ST
- 1.5 ml sterile endotoxin-free water
Catalog code: tlrl-flic-50
- 50 μg Rec FLA-ST
- 10 ml sterile endotoxin-free water
Recombinant flagellin is shipped at room temperature
Stored at -20°C.
Upon resuspension, prepare aliquots of recFLA-ST and store at -20°C.
Resuspended product is stable 6 months at -20°C when properly stored.
Avoid repeated freeze-thaw cycles.
Description
Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species. This proinflammatory molecule is present in both Gram-negative and Gram-positive bacteria. It is recognized by distinct types of pattern recognition receptors (PRRs).
Extracellular flagellin is recognized by surface localized Toll-like receptor 5 (TLR5), which promotes the activation of NF-κB and the subsequent production of cytokines [1, 2]. Flagellin is also recognized by the cytosolic NOD-like receptors (NLRs) NAIP5/NAIP6 sensors of the NLRC4 inflammasome [3, 4]. Flagellin monomers are translocated into the host cell cytosol by a mechanism that requires bacterial secretion systems. This triggers the formation of a NAIP-NLRC4 inflammasome leading to caspase-1-mediated secretion of IL-1β and IL-18 [3, 4].
1. Song WS. et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Sci Rep. 7:40878.
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
3. Duncan JA. & Canna SW., 2018. The NLRC4 Inflammasome. Immunol Rev. 281(1):115-123.
4. Zhao Y. et al., 2011. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 477(7366):596-600.