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hTLR5-Fc

hTLR5-Fc Unit size Cat. code Docs Qty Price
Soluble ectodomain of TLR5
50 µg
fc-htlr5
+-
$391.00

Toll-like receptor 5 (TLR5) is a type-1 transmembrane receptor comprising an N-terminal extracellular leucine rich repeat domain and a C-terminal intracellular TIR signaling domain.

The soluble TLR5 receptor, hTLR5-Fc, was generated by fusing the N-terminal extracellular domain of human TLR5 (aa 21-639) to the N-terminus of an engineered Fc region of human IgG1 with a 2 amino acid linker.

The hTLR5-hFc fusion has an apparent molecular weight of 110 kDa on SDS-PAGE.

Fc-hTLR5 is expressed in CHO cells and purified by protein G affinity chromatography.

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Specifications

Applications: Neutralization, receptor binding assays

Formulation: 0.2 µm filtered solution in phosphate buffer with glycine, saccharose and stabilizing agents

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Contents

  • 50 µg lyophilized Fc-hTLR5

room temperature Product is shipped at room temperature.

store Store lyophilized product at -20°C.

stable Product is stable for at least 1 year.

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Details

Neutralization activity of hTLR5-Fc

Neutralization activity of hTLR5-Fc:

Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.

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Citations

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