hTLR5-Fc
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Cat.code:
fc-htlr5-2
- Documents
ABOUT
Soluble ectodomain of human TLR5 - TLR5 inhibitor
Toll-like receptor 5 (TLR5) is a Type I transmembrane receptor comprising an N-terminal extracellular leucine-rich repeat domain and a C-terminal intracellular TIR signaling domain.
The soluble TLR5 receptor, hTLR5-Fc, was generated by fusing the N-terminal extracellular domain of human TLR5 (aa 21-639) to the N-terminus of the Fc region of human IgG1 with a 2 amino acid linker.
The hTLR5-hFc fusion has an apparent molecular weight of 110 kDa on SDS-PAGE.
Fc-hTLR5 is expressed in CHO cells and purified by protein G affinity chromatography.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Phosphate buffer with glycine, saccharose and stabilizing agents
Negative (tested using EndotoxDetect™ assay)
Neutralization, Receptor binding assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:hTLR5-Fc
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Cat code:fc-htlr5-2
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Quantity:2 x 50 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Neutralization activity of hTLR5-Fc:
Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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