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Soluble ectodomain of TLR5
Soluble ectodomain of human TLR5 - TLR5 inhibitor
Toll-like receptor 5 (TLR5) is a type-1 transmembrane receptor comprising an N-terminal extracellular leucine-rich repeat domain and a C-terminal intracellular TIR signaling domain.
The soluble TLR5 receptor, hTLR5-Fc, was generated by fusing the N-terminal extracellular domain of human TLR5 (aa 21-639) to the N-terminus of an engineered Fc region of human IgG1 with a 2 amino acid linker.
The hTLR5-hFc fusion has an apparent molecular weight of 110 kDa on SDS-PAGE.
Fc-hTLR5 is expressed in CHO cells and purified by protein G affinity chromatography.Back to the top
Applications: Neutralization, receptor binding assays
Formulation: 0.2 µm filtered solution in phosphate buffer with glycine, saccharose and stabilizing agentsBack to the top
- 50 µg of lyophilized Fc-hTLR5
Product is shipped at room temperature.
Store lyophilized product at -20°C.Back to the top
Neutralization activity of hTLR5-Fc:
Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.