hTLR5-Fc
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Cat.code:
fc-htlr5-2
- Documents
ABOUT
Soluble ectodomain of human TLR5 - TLR5 inhibitor
Toll-like receptor 5 (TLR5) is a Type I transmembrane receptor comprising an N-terminal extracellular leucine-rich repeat domain and a C-terminal intracellular TIR signaling domain.
The soluble TLR5 receptor, hTLR5-Fc, was generated by fusing the N-terminal extracellular domain of human TLR5 (aa 21-639) to the N-terminus of the Fc region of human IgG1 with a 2 amino acid linker.
The hTLR5-hFc fusion has an apparent molecular weight of 110 kDa on SDS-PAGE.
Fc-hTLR5 is expressed in CHO cells and purified by protein G affinity chromatography.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
100 μg/ml in water
Phosphate buffer, glycine, saccharose, stabilizing agents
Negative (tested using EndotoxDetect™ assay)
Neutralization, receptor binding assays
Cellular neutralization assays
Each lot is functionally tested and validated.
Do not vortex.
CONTENTS
Contents
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Product:hTLR5-Fc
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Cat code:fc-htlr5-2
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Quantity:2 x 50 µg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
Neutralization activity of hTLR5-Fc:
Increasing concentrations of hTLR5-Fc, anti-FLA-ST (antibody against S. typhimurium flagellin) or anti-hTLR5 (antibody against human TLR5) were pre-incubated with 5 µg FLA-ST (S. typhimurium flagellin) prior to the addition of HEK-Blue™ hTLR5 cells, which express the human TLR5 gene and an NF-kB-inducible SEAP gene.
After 24h incubation, TLR5-induced NF-kB activation was assessed by measuring the levels of SEAP using the QUANTI-Blue™ assay.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
Need a CoA ?
