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Fc-mDectin-1a

Fc-mDectin-1a Unit size Cat. code Docs Qty Price
Soluble murine Dectin-1 receptor
50 µg
fc-mdec1a
+-
$391.00

Fc-mDectin-1a is a soluble murine Dectin-1 receptor constructed by fusing the C-terminal extracellular domain of mouse Dectin-1a (aa 67-244) to the C-terminus of an engineered human IgG1 Fc domain with a 10 amino acid linker.

Fc-mDectin-1a has an apparent molecular weight of ~55 kDa on SDS-PAGE.

Fc-mDectin-1a is expressed in CHO cells and purified by protein G affinity chromatography.

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Specifications

Applications: Receptor binding assays, neutralization

Formulation: 0.2 µm filtered solution in phosphate buffer with glycine, saccharose and stabilizing agents

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Contents

  • 50 µg lyophilized Fc-mDectin-1a

room temperature Product is shipped at room temperature.

store Store lyophilized product at -20°C.

stable Product is stable for at least 1 year.

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Details

b-Glucan receptor binding assay

β-Glucan receptor binding assay:
96-well plates were coated with 0.5 µg of various β-glucans and incubated with 1 μg/ml Fc-hDectin-1a or Fc-mDectin-1a.
After 2 hours, an anti-IgG secondary antibody conjugated to alkaline phosphatase was added.
β-Glucan-Dectin-1 binding was assessed using the QUANTI-Blue™ assay.

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