Fc-hDectin-1a
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Cat.code:
fc-hdec1a-2
- Documents
ABOUT
Soluble human Dectin-1 receptor
Fc-hDectin-1a is a soluble human Dectin-1 receptor constructed by fusing the C-terminal extracellular domain of human Dectin-1a (aa 67-247) to the C-terminus of an engineered human IgG1 Fc domain with a 10 amino acid linker.
Fc-hDectin-1a has an apparent molecular weight of ~55 kDa on SDS-PAGE.
Fc-hDectin-1a is expressed in CHO cells and purified by protein G affinity chromatography.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Phosphate buffered saline (pH 7.4) containing 10% v/v FBS
Receptor binding assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:Fc-hDectin-1a
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Cat code:fc-hdec1a-2
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Quantity:2 x 50 µg
1.5 ml endotoxin-free water
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
β-Glucan receptor binding assay:
96-well plates were coated with 0.5 µg of various β-glucans and incubated with 1 μg/ml Fc-hDectin-1a or Fc-mDectin-1a.
After 2 hours, an anti-IgG secondary antibody conjugated to alkaline phosphatase was added.
β-Glucan-Dectin-1 binding was assessed using the QUANTI-Blue™ assay.
DOCUMENTS
Documents
Technical Data Sheet
Safety Data Sheet
Certificate of analysis
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