Invivogen
Menu

Fc-hDectin-1a

Fc-hDectin-1a Unit size Cat. code Docs Qty Price
Soluble human Dectin-1 receptor
50 µg
fc-hdec1a
+-
$391.00

Fc-hDectin-1a is a soluble human Dectin-1 receptor constructed by fusing the C-terminal extracellular domain of human Dectin-1a (aa 67-247) to the C-terminus of an engineered human IgG1 Fc domain with a 10 amino acid linker.

Fc-hDectin-1a has an apparent molecular weight of ~55 kDa on SDS-PAGE.

Fc-hDectin-1a is expressed in CHO cells and purified by protein G affinity chromatography.

Back to the top

Specifications

Applications: Receptor binding assays, neutralization

Formulation: 0.2 µm filtered solution in phosphate buffered saline (pH 7.4) containing 10% v/v FBS

Back to the top

Contents

  • 50 µg lyophilized Fc-hDectin-1a

room temperature Product is shipped at room temperature.

store Store lyophilized product at -20°C.

stable Product is stable for at least 1 year.

Back to the top

Details

b-Glucan receptor binding assay

β-Glucan receptor binding assay:
96-well plates were coated with 0.5 µg of various β-glucans and incubated with 1 μg/ml Fc-hDectin-1a or Fc-mDectin-1a.
After 2 hours, an anti-IgG secondary antibody conjugated to alkaline phosphatase was added.
β-Glucan-Dectin-1 binding was assessed using the QUANTI-Blue™ assay.

Back to the top

Customer Service
& Technical Support

CAPTCHA
In order to prevent automated spam submissions, please answer the following question:
4 + 1 =
Solve this simple math problem and enter the result. E.g. for 1+3, enter 4.
Shopping cart is empty