Anti-mouse TLR2 (clone T2.5) Detection and Neutralizing mAb
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Anti-mouse/human TLR2 mAb (clone T2.5)
Detection and neutralizing antibody for mouse and human TLR2 (clone T2.5)
MAb mTLR2 (clone T2.5) is a monoclonal antibody that reacts with mouse Toll-like receptor 2 (TLR2, CD282). MAb mTLR2 is an antagonistic antibody.
It has been tested by flow cytometric analysis of RAW264.7 cells.
The antibody is cross reactive with human TLR2. It was generated using recombinant DNA technology, produced in CHO (Chinese hamster ovary) cells, and purified by affinity chromatography.Back to the top
Clonality: Monoclonal antibody
Specificity: Human & mouse TLR2
Isotype: Mouse IgG1, kappa
Source: CHO cells
Formulation: 0.2 mm filtered solution in sodium phosphate buffer with glycine, saccharose and stabilizing agents
Tested applications: Neutralization of mouse TLR2-induced cellular activation. This antibody can also be used for detection using flow cytometry, immunohistology on frozen tissue sections and immunoprecipitation.Back to the top
- 100 μg purified monoclonal anti-mTLR2 antibody (MAb-mTLR2) provided lyophilized.
Product is shipped at room temperature.
Store lyophilized MAb-mTLR2 at -20°C.
Product is stable for 1 year. Resuspended MAb-mTLR2 should be stored at -20°C for 1 year.Back to the top
Toll-Like receptors (TLRs) play a critical role in early innate immunity toinvading pathogens by sensing microorganisms. These evolutionary conserved receptors recognize highly conserved structural motifs only expressed by microbial pathogens, called pathogen-associated microbial patterns (PAMPs). Stimulation of TLRs by PAMPs initiates a signaling cascade leading to the secretion of proinflammatory cytokines following NF-κB activation. To date ten human and twelve murine TLRs have been characterized, TLR1 to TLR10 in humans, and TLR1 to TLR9, TLR11, TLR12 and TLR13 in mice, the homolog of TLR10 being a pseudogene.
TLR2 is involved in the recognition of a wide array of microbial molecules. TLR2 recognizes lipoteichoic acid and lipoprotein from gram- positive bacteria, lipoarabinomannan from mycobacteria, and zymosan from yeast cell wall. Moreover, TLR2 participates in the recognition of some types of LPS. TLR2 is known to heterodimerize with other TLRs, a property believed to extend the range of microbial molecules that TLR2 can recognize. TLR2 cooperates with TLR6 in response to diacylated mycoplasmal lipopeptide , and associates with TLR1 to recognize triacylated lipopetides . Furthermore, pathogen recognition by TLR2 is strongly enhanced by CD14 .
1. Girard R et al., 2003. Lipopolysaccharides from Legionella and Rhizobium stimulate mouse bone marrow granulocytes via Toll-like receptor 2. J Cell Sci. 116(Pt 2):293-302.
2. Ozinsky A. et al., 2000. The repertoire for pattern recognition of pathogens by the innate immune system is defined by cooperation between toll-like receptors. Proc Natl Acad Sci USA. 97(25):13766-71.
3. Lotz S. et al., 2004. Highly purified lipoteichoic acid activates neutrophil granulocytes and delays their spontaneous apoptosis via CD14 and TLR2. J Leukoc Biol. 75(3):467-77.