|HEK-Blue™ mTLR2 cells||Unit size||Cat. code||Docs||Qty||Price|
murine TLR2-expressing HEK 293 cells
3-7 x 10e6 cells
Murine TLR2 / NF-κB / SEAP reporter HEK293 cells
HEK-Blue™-mTLR2 cells were obtained by co-transfection of the murine TLR2 and SEAP (secreted embryonic alkaline phosphatase) reporter genes into HEK293 cells.
The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.
Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induces the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ mTLR2 cells is HEK-Blue™ Null2 cells.
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Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.
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- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml 250X HEK-Blue™ Selection
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
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