Invivogen
Menu

HEK-Blue™ mTLR2

HEK-Blue™ mTLR2 cells Unit size Cat. code Docs Qty Price
murine TLR2-expressing HEK 293 cells
3-7 x 10e6 cells
hkb-mtlr2
+-
$1,408.00

You may also need : HEK-Blue™ Selection | View more associated products

Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

Murine TLR2 / NF-κB–SEAP reporter HEK293 cells

HEK-Blue™-mTLR2 cells were obtained by co-transfection of the murine TLR2 and SEAP (secreted embryonic alkaline phosphatase) reporter genes into HEK293 cells.

The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.

Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induces the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ mTLR2 cells is HEK-Blue™ Null2 cells.

 

Read our review Read our review on TLR2

Back to the top

Specifications

Antibiotic resistance: blasticidin, hygromycin, Zeocin®.

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.

Guaranteed mycoplasma-free.

 

This product is covered by a Limited Use License (See Terms and Conditions).

Back to the top

Contents

Dry ice shipping Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

Back to the top

FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

Back to the top
Customer Service
& Technical Support
Shopping cart is empty