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Mouse TLR2 Reporter HEK293 Cells (NF-κB)

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HEK-Blue™ mTLR2 cells

Murine TLR2 expressing HEK293 reporter cells (NF-κB pathway)

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3-7 x 10e6 cells

hkb-mtlr2
+-
$1,457

NF-κB–SEAP reporter HEK293 cells expressing mouse TLR2

HEK-Blue™-mTLR2 cells were obtained by co-transfection of the murine TLR2 and SEAP (secreted embryonic alkaline phosphatase) reporter genes into HEK293 cells.

The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.

Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induces the production of SEAP.

Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.

The parental cell line of HEK-Blue™ mTLR2 cells is HEK-Blue™ Null2 cells.

 

Read our review Read our review on TLR2

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Specifications

Antibiotic resistance: blasticidin, hygromycin, Zeocin®.

Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.

Guaranteed mycoplasma-free.

 

This product is covered by a Limited Use License (See Terms and Conditions).

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Contents

Dry ice shipping Shipped on dry ice (Europe, USA, Canada and some areas in Asia)

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FAQ Cell Lines

Visit our FAQ Any questions about our cell lines ? Visit our frequently asked questions page

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Citations

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Notification:  This product is for internal research use only. Additional rights may be available. Please visit InvivoGen’s Terms and Conditions.

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