Mouse TLR2 Reporter HEK293 Cells (NF-κB)
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HEK-Blue™ mTLR2 Cells Murine TLR2 expressing HEK293 reporter cells (NF-κB pathway) |
Show product |
3-7 x 10e6 cells |
hkb-mtlr2
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HEK-Blue™ mTLR2 vial Additional cell vial |
Show product |
3-7 x 10e6 cells |
hkb-mtlr2-av
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Notification:
Reference #hkb-mtlr2-av can only be ordered together with reference #hkb-mtlr2.
NF-κB–SEAP reporter HEK293 cells expressing mouse TLR2
HEK-Blue™-mTLR2 cells were obtained by co-transfection of the murine TLR2 and SEAP (secreted embryonic alkaline phosphatase) reporter genes into HEK293 cells.
The SEAP reporter gene is placed under the control of the IL-12 p40 minimal promoter fused to five NF-κB and AP-1-binding sites.
Additionally, the CD14 co-receptor gene was transfected into these cells to enhance the TLR2 response. Stimulation with a TLR2 ligand activates NF-κB and AP-1 which induces the production of SEAP.
Levels of SEAP can be easily determined with HEK-Blue™ Detection, a cell culture medium that allows for real-time detection of SEAP.
The parental cell line of HEK-Blue™ mTLR2 cells is HEK-Blue™ Null2 cells.
Read our review on TLR2
Specifications
Antibiotic resistance: blasticidin, hygromycin, Zeocin®.
Growth medium: DMEM, 4.5 g/l glucose, 2 mM L-glutamine, 10% (v/v) fetal bovine serum, 100 U/ml penicillin, 100 μg/ml streptomycin, 100 μg/ml Normocin™.
Guaranteed mycoplasma-free
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- 1 vial containing 3-7 x 106 cells
- 2 x 1 ml 250X HEK-Blue™ Selection
- 1 ml Normocin™ (50 mg/ml)
- 1 pouch of HEK-Blue™ Detection (cell culture medium for real-time detection of SEAP)
Shipped on dry ice (Europe, USA, Canada and some areas in Asia)
FAQ Cell Lines
Any questions about our cell lines ? Visit our frequently asked questions page
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Disclaimer: These cells are for internal research use only and are covered by a Limited Use License (See Terms and Conditions). Additional rights may be available.