|Anti-hCD14-IgG||Unit size||Cat. code||Docs||Qty||Price|
Neutralizing IgG monoclonal antibody to human CD14 (D3B8)
Neutralizing IgG monoclonal antibody to human CD14
Anti-hCD14-IgG is a chimeric monoclonal antibody specific for human CD14. It has been selected for its ability to efficiently neutralize the biological activity of TLR2 or TLR4.
Anti-hCD14-IgG was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.Back to the top
Clonality: Monoclonal antibody
Applications: Neutralizing TLR2 or TLR4-induced cellular activation. It can also be used for flow cytometry.
Specificity: Human CD14
Isotype: Human IgG1
Formulation: 0.2 μm filtered solution in sodium phosphate buffer with glycine, saccharose and stabilizing agentsBack to the top
- 100 μg purified anti-hCD14-IgG provided azide-free and lyophilized.
Product is shipped at room temperature.
Store lyophilized antibody at -20 °C.
Product is stable for 1 year.Back to the top
CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor . CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 . CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections .
Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.
Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.
Aderem A. & Ulevitch R.J. , 2000. Tolllike receptors in the induction of the innate immune response. Nature. 406(6797):782-7.