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Anti-hCD14-IgG

Anti-hCD14-IgG Unit size Cat. code Docs Qty Price
Neutralizing IgG monoclonal antibody to human CD14 (D3B8)
100 µg
mabg-hcd14
+-
$325.00

Neutralizing IgG monoclonal antibody to human CD14

Anti-hCD14-IgG is a chimeric monoclonal antibody specific for human CD14. It has been selected for its ability to efficiently neutralize the biological activity of TLR2 or TLR4.

Anti-hCD14-IgG was generated by recombinant DNA technology. It has been produced in CHO cells and purified by affinity chromatography.

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Specifications

Clonality: Monoclonal antibody

Applications: Neutralizing TLR2 or TLR4-induced cellular activation. It can also be used for flow cytometry.

Specificity: Human CD14

Clone: D3B8

Isotype: Human IgG1

Formulation: 0.2 μm filtered solution in sodium phosphate buffer with glycine, saccharose and stabilizing agents

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Contents

  • 100 μg purified anti-hCD14-IgG provided azide-free and lyophilized.

room temperature Product is shipped at room temperature.

store Store lyophilized antibody at -20 °C.

stable Product is stable for 1 year.

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Description

CD14 is a glycosylphosphatidylinositol (GPI)-anchored membrane protein which acts as a bacterial pattern recognition receptor [1]. CD14 is found on cells derived from the monocyte/macrophage lineage, as well as neutrophils and B lymphocytes. CD14 serves as a member of the heteromeric lipopolysaccharide (LPS) receptor complex that also contains TLR4 and MD2 [2]. CD14 binds LPS but is not capable of initiating a transmembrane activation signal since it does not contain a cytoplasmic domain. Upon LPS binding, CD14 physically associates with TLR4 which in turn transduces the signal. CD14 was also shown to interact with TLR2 in response to various microbial infections [3].

 

Pugin J. et al., 1994. CD14 is a pattern recognition receptor. Immunity. 1(6):509-16.
Da Silva Correia J. et al., 2001. Lipopolysaccharide is in close proximity to each of the proteins in its membrane receptor complex. transfer from CD14 to TLR4 and MD-2. J Biol Chem. 276(24): 21129-35.
Aderem A. & Ulevitch R.J. , 2000. Tolllike receptors in the induction of the innate immune response. Nature. 406(6797):782-7.

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