|Anti-Flagellin FliC||Unit size||Cat. code||Docs||Qty||Price|
S. typhimurium flagellin FliC Detection antibody - Mouse monoclonal IgG1 (X5A12)
Anti-Flagellin FliC is a mouse monoclonal antibody to Salmonella typhimurium flagellin. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species.
Flagellin is recognized by TLR5  and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex .
This MAb was generated by immunizing by DNA vaccination Balb/c mice with the fliC gene and screened by ELISA using purified flagellin (FLA-ST). The antibody was purified by Protein G affinity chromatography.
1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.
Clonality: Monoclonal antibody
Applications: Western blotting; to detect purified or recombinant S. typhimurium flagellin
Specificity: Purified or recombinant S. typhimurium flagellin
Isotype: Mouse IgG1
Formulation: 0.2 μm filtered solution in PBS with 5% saccharoseBack to the top
- 100 μg purified anti-flagellin FliC provided azide-free and lyophilized
Product is shipped at room temperature.
Store lyophilized antibody at -20°C.
Product is stable for 6 months. - Reconstituted antibody is stable 1 month when stored at 4°C and 6 months when aliquoted and stored at -20°C.
Avoid repeated freeze- thaw cycles.Back to the top