Anti-Flagellin
Anti-Flagellin FliC | Unit size | Cat. code | Docs | Qty | Price |
---|---|---|---|---|---|
S. typhimurium flagellin FliC Detection antibody - Mouse monoclonal IgG1 (X5A12) |
100 µg |
mabg-flic |
You may also need : Mouse Control IgG1 | View more associated products ▼
S. typhimurium flagellin FliC Detection antibody - Mouse monoclonal IgG1 (X5A12)
Anti-Flagellin FliC is a mouse monoclonal antibody to Salmonella typhimurium flagellin. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species.
Flagellin is recognized by TLR5 [1] and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex [2].
This MAb was generated by immunizing by DNA vaccination Balb/c mice with the fliC gene and screened by ELISA using purified flagellin (FLA-ST).
This monoclonal antibody was produced in hybridoma cells and purified by affinity chromatography protein G.
References:
1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.
Specifications
Clonality: Monoclonal antibody
Specificity: Purified or recombinant S. typhimurium flagellin
Clone: X5A12
Isotype: Mouse IgG1, kappa
Source: Hybridoma cells
Formulation: 0.2 μm filtered solution in PBS with 5% saccharose
Applications: Detection via ELISA and western blotting; to detect purified or recombinant S. typhimurium flagellin
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- 100 μg purified anti-flagellin FliC provided azide-free and lyophilized
Product is shipped at room temperature.
Store lyophilized antibody at -20°C.
Product is stable for 6 months. - Reconstituted antibody is stable 1 month when stored at 4°C and 6 months when aliquoted and stored at -20°C.
Avoid repeated freeze- thaw cycles.