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S. typhimurium flagellin FliC Detection antibody (clone X5A12) - Mouse monoclonal IgG1
Monoclonal antibody against S. typhimurium flagellin
Anti-Flagellin FliC (clone X5A12) is a monoclonal mouse IgG1 antibody against flagellin from Salmonella typhimurium. This antibody was generated by DNA immunization of Balb/c mice with the fliC gene. It has been produced in hybridomas and purified by affinity chromatography.
Anti-Flagellin FliC was screened for its ability to bind purified flagellin from S. typhimurium (FLA-ST) using ELISA. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species. Flagellin is recognized by TLR5  and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex .
- Reacts with purified and recombinant flagellin from S. typhimurium
- Provided azide-free
- Each lot is functionally tested
1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.
Clonality: Monoclonal antibody
Specificity: Purified or recombinant flagellin from S. typhimurium
Isotype: Mouse IgG1, kappa
Source: Hybridoma cells
Formulation: 0.2 µm filtered solution in Tris HCl buffer with saccharose, glycine, and stabilizing agents
Tested applications: Detection antibody for Western blot and ELISA
- The antibody has been validated by ELISA.
- The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.
- 100 μg of purified Anti-flagellin FliC provided azide-free and lyophilized
Product is shipped at room temperature.
Upon receipt, store lyophilized antibody at -20°C.
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Flagellin is the major component of the bacterial flagellar filament, which confers motility to a wide range of bacterial species. This proinflammatory molecule is present in both Gram-negative and Gram-positive bacteria. It is recognized by distinct types of pattern recognition receptors (PRRs).
Extracellular flagellin is recognized by surface-localized Toll-like receptor 5 (TLR5), which promotes the activation of NF-κB and the subsequent production of cytokines [1, 2]. Flagellin is also recognized by the cytosolic NOD-like receptors (NLRs) NAIP5/NAIP6 sensors of the NLRC4 inflammasome [3, 4]. Flagellin monomers are translocated into the host cell cytosol by a mechanism that requires bacterial secretion systems. This triggers the formation of an NAIP-NLRC4 inflammasome leading to caspase-1-mediated secretion of IL-1β and IL-18 [3, 4].
1. Song WS. et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Sci Rep. 7:40878.
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
3. Duncan JA. & Canna SW., 2018. The NLRC4 Inflammasome. Immunol Rev. 281(1):115-123.
4. Zhao Y. et al., 2011. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 477(7366):596-600.