Bacterial Flagellin Antibody

Mouse IgG1 (clone X5A12) - Hybridoma

ABOUT

Monoclonal antibody against S. typhimurium flagellin

Anti-Flagellin FliC (clone X5A12) is a monoclonal mouse IgG1 antibody against flagellin from Salmonella typhimurium. This antibody was generated by DNA immunization of Balb/c mice with the fliC gene.  It has been produced in hybridomas and purified by affinity chromatography.
Anti-Flagellin FliC was screened for its ability to bind purified flagellin from S. typhimurium (FLA-ST) using ELISA. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species. Flagellin is recognized by TLR5 [1] and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex [2].

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Key features:

  • Reacts with purified and recombinant flagellin from S. typhimurium
  • Provided azide-free
  • Each lot is functionally tested

 

References:

1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.

All products are for research use only, and not for human or veterinary use.

S. typhimurium Flagellin Detection with Anti-Flagellin FliC
S. typhimurium Flagellin Detection with Anti-Flagellin FliC

SPECIFICATIONS

Specifications

Target

Flagellin FliC

Applications

Western blot (tested), ELISA (tested)

Antibody applications
WB
ELISA
Isotype
mIgG1
Recommended isotype control
Mouse IgG1 Isotype Control Antibody
Clone
X5A12
Source
Hybridoma
Purification
Affinity chromatography
Formulation buffer

Sodium phosphate buffer with glycine, saccharose, and stabilizing agents

Preservative
Azide-free
Reconstitution buffer
Sterile water (not provided)
Sterility

0.2 µm filtration

Quality control

Each lot is functionally tested and validated.

CONTENTS

Contents

  • Product: 
    Anti-Flagellin FliC
  • Cat code: 
    mabg-flic-2
  • Quantity: 
    2 x 100 µg

Shipping & Storage

  • Shipping method:  Room temperature

    • Storage:

    • -20°C
    Stability: Reconstituted antibody is stable for 1 month at 4°C and for 1 year at -20°C. Avoid repeated freeze-thaw cycles.

    Caution:

    • Avoid repeated freeze-thaw cycles

Details

Flagellin is the major component of the bacterial flagellar filament, which confers motility to a wide range of bacterial species. This proinflammatory molecule is present in both Gram-negative and Gram-positive bacteria. It is recognized by distinct types of pattern recognition receptors (PRRs).
Extracellular flagellin is recognized by surface-localized Toll-like receptor 5 (TLR5), which promotes the activation of NF-κB and the subsequent production of cytokines [1, 2]. Flagellin is also recognized by the cytosolic NOD-like receptors (NLRs) NAIP5/NAIP6 sensors of the NLRC4 inflammasome [3, 4]. Flagellin monomers are translocated into the host cell cytosol by a mechanism that requires bacterial secretion systems. This triggers the formation of an NAIP-NLRC4 inflammasome leading to caspase-1-mediated secretion of IL-1β and IL-18 [3, 4]. 

 

1. Song WS. et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Sci Rep. 7:40878.
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
3. Duncan JA. & Canna SW., 2018. The NLRC4 Inflammasome. Immunol Rev. 281(1):115-123.
4. Zhao Y. et al., 2011. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 477(7366):596-600.

DOCUMENTS

Documents

Anti-Flagellin FliC

Technical Data Sheet

Safety Data Sheet

Certificate of analysis

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