Anti-Flagellin FliC Unit size Cat. code Docs Qty Price
S. typhimurium flagellin FliC Detection antibody - Mouse monoclonal IgG1 (X5A12)
100 µg

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S. typhimurium flagellin FliC Detection antibody - Mouse monoclonal IgG1 (X5A12)

Anti-Flagellin FliC is a mouse monoclonal antibody to Salmonella typhimurium flagellin. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species.

Flagellin is recognized by TLR5 [1] and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex [2].

This MAb was generated by immunizing by DNA vaccination Balb/c mice with the fliC gene and screened by ELISA using purified flagellin (FLA-ST). 

This monoclonal antibody was produced in hybridoma cells and purified by affinity chromatography protein G.




1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.

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Clonality: Monoclonal antibody

Specificity: Purified or recombinant S. typhimurium flagellin

Clone: X5A12

Isotype: Mouse IgG1, kappa

Source: Hybridoma cells

Formulation: 0.2 μm filtered solution in PBS with 5% saccharose

Applications: Detection via ELISA and western blotting; to detect purified or recombinant S. typhimurium flagellin

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  • 100 μg purified anti-flagellin FliC provided azide-free and lyophilized

room temperature Product is shipped at room temperature.

store Store lyophilized antibody at -20°C.

stability Product is stable for 6 months. - Reconstituted antibody is stable 1 month when stored at 4°C and 6 months when aliquoted and stored at -20°C.

Alert Avoid repeated freeze- thaw cycles.

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