Anti-Flagellin

S. typhimurium flagellin FliC Detection antibody - Mouse monoclonal IgG1 (X5A12)

Anti-Flagellin FliC is a mouse monoclonal antibody to Salmonella typhimurium flagellin. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species.

Flagellin is recognized by TLR5 [1] and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex [2].

This MAb was generated by immunizing by DNA vaccination Balb/c mice with the fliC gene and screened by ELISA using purified flagellin (FLA-ST). 

This monoclonal antibody was produced in hybridoma cells and purified by affinity chromatography protein G.

References:

1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.

Specifications

Clonality: Monoclonal antibody

Specificity: Purified or recombinant S. typhimurium flagellin

Clone: X5A12

Isotype: Mouse IgG1, kappa

Source: Hybridoma cells

Formulation: 0.2 μm filtered solution in PBS with 5% saccharose

Applications: Detection via ELISA and western blotting; to detect purified or recombinant S. typhimurium flagellin

Contents

• 100 μg purified anti-flagellin FliC provided azide-free and lyophilized

Product is shipped at room temperature.

Store lyophilized antibody at -20°C.

Product is stable for 6 months. - Reconstituted antibody is stable 1 month when stored at 4°C and 6 months when aliquoted and stored at -20°C.

Avoid repeated freeze- thaw cycles.