Anti-Flagellin

Monoclonal antibody against S. typhimurium flagellin

S. typhimurium Flagellin Detection with Anti-Flagellin FliC

Anti-Flagellin FliC (clone X5A12) is a monoclonal mouse IgG1 antibody against flagellin from Salmonella typhimurium. This antibody was generated by DNA immunization of Balb/c mice with the fliC gene.  It has been produced in hybridomas and purified by affinity chromatography.
Anti-Flagellin FliC was screened for its ability to bind purified flagellin from S. typhimurium  (FLA-ST) using ELISA. Flagellin is the major component of the bacterial flagellar filament, which confers motility on a wide range of bacterial species. Flagellin is recognized by TLR5 [1] and induces the activation of NF-κB and the production of cytokines and nitric oxide depending on the nature of the TLR5 signaling complex [2].

More details

Key features:

• Reacts with purified and recombinant flagellin from S. typhimurium  
• Provided azide-free
• Each lot is functionally tested

References:

1. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
2. Mizel SB. et al., 2003. Induction of macrophage nitric oxide production by Gram-negative flagellin involves signaling via heteromeric Toll-like receptor 5/Toll-like receptor 4 complexes. J Immunol. 170(12):6217-23.

Specifications

Clonality: Monoclonal antibody

Specificity: Purified or recombinant flagellin from S. typhimurium 

Clone: X5A12

Isotype: Mouse IgG1, kappa

Source: Hybridoma cells

Formulation: 0.2 µm filtered solution in Tris HCl buffer with saccharose, glycine, and stabilizing agents

Tested applications: Detection antibody for Western blot and ELISA 

Quality control:
   - The antibody has been validated by ELISA.
   - The absence of bacterial contamination (e.g. lipoproteins and endotoxins) has been confirmed using HEK-Blue™ TLR2 and HEK-Blue™ TLR4 cells.

Contents

• 100 μg of purified Anti-flagellin FliC provided azide-free and lyophilized

 Product is shipped at room temperature.

Upon receipt, store lyophilized antibody at -20°C.

Details

Flagellin is the major component of the bacterial flagellar filament, which confers motility to a wide range of bacterial species. This proinflammatory molecule is present in both Gram-negative and Gram-positive bacteria. It is recognized by distinct types of pattern recognition receptors (PRRs).
Extracellular flagellin is recognized by surface-localized Toll-like receptor 5 (TLR5), which promotes the activation of NF-κB and the subsequent production of cytokines [1, 2]. Flagellin is also recognized by the cytosolic NOD-like receptors (NLRs) NAIP5/NAIP6 sensors of the NLRC4 inflammasome [3, 4]. Flagellin monomers are translocated into the host cell cytosol by a mechanism that requires bacterial secretion systems. This triggers the formation of an NAIP-NLRC4 inflammasome leading to caspase-1-mediated secretion of IL-1β and IL-18 [3, 4]. 

1. Song WS. et al., 2017. A conserved TLR5 binding and activation hot spot on flagellin. Sci Rep. 7:40878.
2. Hayashi F. et al., 2001. The innate immune response to bacterial flagellin is mediated by Toll-like receptor 5. Nature. 410(6832):1099-103.
3. Duncan JA. & Canna SW., 2018. The NLRC4 Inflammasome. Immunol Rev. 281(1):115-123.
4. Zhao Y. et al., 2011. The NLRC4 inflammasome receptors for bacterial flagellin and type III secretion apparatus. Nature. 477(7366):596-600.