FICZ
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AhR agonist
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Cat.code:
tlrl-ficz
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ABOUT
Tryptophan-derived agonist of AhR
FICZ (6-Formylindolo[3,2-b]carbazole) is a highly potent ligand of the cytosolic aryl hydrocarbon receptor (AhR) transcription factor.
The presence of FICZ-derived metabolites in human urine points FICZ to be an endogenous AhR agonist, but which requires diet-derived tryptophan (Trp) amino acid [1]. FICZ synthesis results from Trp conversion upon UV-dependent photo-oxidation or H2O2-mediated oxidative stress [2,3].
InvivoGen's FICZ is a high-quality product (>95% purity) and is guaranteed free of bacterial contamination. Each lot of FICZ is functionally tested in cellular assays using HepG2-Lucia™ AhR and HT29-Lucia™ AhR reporter cells.
CH-223191, an AhR antagonist is also available.
Read our review on AhR's key role in the intestinal microbiota and immunity.
References:
1. Wincent E. et al., 2009. The suggested physiologic aryl hydrocarbon receptor activator and cytochrome P4501 substrate 6-formylindolo[3,2-b]carbazole is present in humans. J. Biol. Chem. 284:2690-96.
2. Rannug A. et al., 1987. Certain photooxidized derivatives of tryptophan bind with very high affinity to the Ah receptor and are likely to be endogenous signal substances. J. Biol. Chem. 262:15422-27.
3. Smirnova A. et al., 2018. Evidence for new light-independent pathways for generation of the endogenous aryl hydorcarbon receptor agonist FICZ. Chem. Res. Toxicol. 29:75-86.
All InvivoGen products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
C19H12N2O
50 ng/ml (0.18 µM) to 5 μg/ml (18 μM)
10 mg/ml (37.17 mM) in DMSO
AhR activation using HepG2-Lucia™ AhR and HT29-Lucia™ AhR reporter cellular assays
Each lot is functionally tested and validated.
CONTENTS
Contents
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Product:FICZ
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Cat code:tlrl-ficz
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Quantity:1 mg
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
Details
AhR Background
The aryl hydrocarbon receptor (AhR) is a ligand-dependent transcriptional factor widely expressed in barrier tissues [2]. AhR plays a key role in gut-microbiota and host immune homeostasis [2]. Besides xenobiotics, including the prototypic AhR agonist 2,3,7,8-tetrachlorodibenzo-p-dioxin (TCDD), a variety of dietary-derived AhR ligands have been identified, many of which are byproducts of tryptophan metabolism [2].
Inactive AhR resides inactive in the cytoplasm within protein complex consisting of Hsp90:XAP2:p23:Src. The AhR canonical genomic signaling pathway occurs as follows: upon ligand binding, the complex undergoes conformational changes and partly translocates into the nucleus. AhR heterodimerizes with AhR nuclear translocator (ARNT) before binding to dioxin response elements (DREs) in the upstream regulatory regions of AhR target genes, such as the cytochrome P450-dependent monooxygenase Cyp1a1 or the AhR repressor (AhRR) [2].
Given the pleiotropic and highly context-dependent nature of AHR signaling, further studies are essential to fully elucidate its complex biological functions and therapeutic potential across a broad range of diseases, including cancer, inflammatory and autoimmune disorders [1-2]. For example, disruptions in AhR signaling have been shown to be involved in the development of diseases such as inflammatory bowel disease (IBD) and atopic dermatitis (AD) [2].
References
1. Karasová M, et al., 2022. Inhibition of Aryl Hydrocarbon Receptor (AhR) Expression Disrupts Cell Proliferation and Alters Energy Metabolism and Fatty Acid Synthesis in Colon Cancer Cells. Cancers (Basel). 14(17):4245
2. Coumoul X, et al., 2026. The aryl hydrocarbon receptor: structure, signaling, physiology and pathology. Signal Transduct Target Ther.11(1):20.
DOCUMENTS
Documents
Technical Data Sheet
Validation Data Sheet
Safety Data Sheet
Certificate of analysis
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