ADP-Heptose (D- & L-isomer)
|ADP-L-Heptose||Unit size||Cat. code||Docs||Qty||Price|
ALPK1 ligand - L isomer
|ADP-Heptose||Unit size||Cat. code||Docs||Price|
ALPK1 Ligand - D isomer
Synthetic ALPK1-TIFA inducers
Bacterial ADP-Heptose is an intermediary sugar in the biosynthesis of lipopolysaccharide (LPS), an essential component of the outer membrane of Gram-negative bacteria. It is generated by a multi-step biosynthesis pathway, in which the final step is the interconversion between two isomers, ADP-D-glycero-β-D-manno-heptose and ADP-L-glycero-β-D-manno-heptose, catalyzed by an epimerase enzyme (e.g. HldD). Importantly, synthetic forms of the D- and L- isomers of ADP-Heptose have been shown to trigger comparable NF-κB-dependent signaling in vitro [1-3].
InvivoGen has synthesized and readily provides both isomers:
• ADP‑L-Heptose– ADP-L-glycero-β-D-manno-heptose (L‑isomer)
• ADP-Heptose – ADP-D-glycero-β-D-manno-heptose (D‑isomer)
Both isomers of ADP-Heptose have been identified as potent PAMPs from Gram-negative bacteria that bind to the cytosolic receptor, ALPK1 [1-3]. By binding to ALPK1, ADP-Heptose triggers the oligomerization of TIFA and the recruitment of TRAF6. Ultimately, this results in the activation of NF-κB and a strong pro-inflammatory response .
- Potent LPS-intermediary metabolite produced by all Gram-negative bacteria
- Activates the cytosolic ALPK1-TIFA signaling pathway
- Easily penetrates the cell wall for delivery to the host cell cytoplasm
InvivoGen's ADP-Heptose (D- isomer) and ADP-L-Heptose are of the highest quality, guaranteed free of bacterial contamination, and have been functionally validated on HEK-Blue™ Null1-v cells. Additionally, to foster research into ADP-Heptose-dependent signaling, InvivoGen provides HEK-Blue™ KO-ALPK1 and HEK-Blue™ KO-TIFA cells.
1. Pfannkuch, L. et al. 2019. ADP heptose, a novel pathogen-associated molecular pattern identified in Helicobacter pylori. FASEB J, fj201802555R.
2. Garcia-Weber, D. et al. 2018. ADP-heptose is a newly identified pathogen-associated molecular pattern of Shigella flexneri. EMBO Rep 19
3. Zhou, P. et al. 2018. Alpha-kinase 1 is a cytosolic innate immune receptor for bacterial ADP-heptose. Nature 561, 122-126.
ADP-Heptose induced NF-κB response. HEK-Blue™ Null1-v, HEK-Blue™ KO‑ALPK1, and HEK-Blue™ KO‑TIFA cells were incubated with increasing concentrations of ADP‑Heptose (0 - 30 µg/ml). After overnight incubation, the NF-κB response was assessed by measuring the activity of SEAP in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent. Data are presented as optical density (OD) at 630 nm (mean ± SEM). The EC50 value is indicated (± std error).
ADP-D-Heptose vs. ADP-L-Heptose NF-κB response. HEK-Blue™ Null1-v cells were incubated with increasing concentrations of either ADP‑D-Heptose or ADP-L-Heptose (0 - 10 µg/ml). After overnight incubation, the NF-κB response was assessed by measuring the activity of SEAP in the supernatant using QUANTI-Blue™ Solution, a SEAP detection reagent. Data are presented as optical density (OD) at 630 nm (mean ± SEM). The EC50 value is indicated (± std error).
Formula: C17H27N5O16P2 . Et3NH (stable form)
Molecular weight: 720.21 g/mol (stable form)
- ADP-Heptose - D isomer
- ADP-L-Heptose - L isomer
Solubility: 10 mg/ml in H2O
Working concentration: 0.01 - 30 µg/ml
- Purity >95% (UHPLC)
- Activation of the ALPK1/TIFA signaling pathway has been confirmed using a cellular assay.
- Absence of bacterial contamination (i.e. endotoxins) has been confirmed using a kinetic chromogenic LAL assay, with an endotoxin level <1 EU/mg
Please note: Each ligand is sold separately. See TDS for the exact contents.
- 250 µg ADP-Heptose or ADP-L-Heptose (provided as a dried powder)
- 1.5 ml endotoxin-free water
ADP-Heptose and ADP-L-Heptose are shipped at room temperature.
Store at -20°C. Upon resuspension, prepare aliquots and store at -20°C.
Resuspended product is stable for 3 months at -20°C.
Avoid repeated freeze-thaw cycles.Back to the top