pDRIVE-mPGK
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Cat.code:
pdrive-mpgk
- Documents
ABOUT
PGK-1 promoter (Ubiquitous) in pDRIVE expression plasmid
Pgk-1 is an X-linked gene encoding 3-phosphoglycerate kinase, an enzyme necessary in every cell for glycolysis.
The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, a potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box [1].
This promoter can efficiently drive high levels of expression of reporter genes (i.e. LacZ and GFP) and therapeutic genes, such as tumor-associated antigenes [2, 3]. Furthermore, in contrast to the CMV promoter, the PGK promoter yields sustained expression [2].
References:
1. Adra C.N. et al., 1987. Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Gene 60(1):65-74.
2. Gerolami R. et al. 2000. Gene transfer to hepatocellular carcinoma: transduction efficacy and transgene expression kinetics by using retroviral and lentiviral vectors. Cancer Gene Ther 7(9):1286-92.
3. Lizee G. et al., 2004. Lentivirus vector-mediated expression of tumor-associated epitopes by human antigen presenting cells. Hum Gene Ther. 15(4):393-404.
All products are for internal research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
in vitro or in vivo transfection
Ubiquitous
in vitro transfection
Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing, Plasmid DNA was purified by ion exchange chromatography
CONTENTS
Contents
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Product:pDRIVE-mPGK
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Cat code:pdrive-mpgk
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Quantity:20 µg
1 ml of Zeocin® (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Technical Data Sheet
Plasmid Map and Sequence
Plasmid Sequence
Safety Data Sheet
Certificate of analysis
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