pDRIVE-PGK-1

PGK-1 promoter (Ubiquitous) in pDRIVE expression plasmid

ABOUT

PGK-1 promoter (Ubiquitous) in pDRIVE expression plasmid

Pgk-1 is an X-linked gene encoding 3-phosphoglycerate kinase, an enzyme necessary in every cell for glycolysis.

The promoter region of the pgk-1 gene is rich in G and C nucleotides and contains five copies of the hexadeoxynucleotide, GGGCGG, a potential binding site for the Sp1 transcription factor, a CCAAT sequence, but no TATA box [1].

This promoter can efficiently drive high levels of expression of reporter genes (i.e. LacZ and GFP) and therapeutic genes, such as tumor-associated antigenes [2, 3]. Furthermore, in contrast to the CMV promoter, the PGK promoter yields sustained expression [2].

 

References:

1. Adra C.N. et al., 1987. Cloning and expression of the mouse pgk-1 gene and the nucleotide sequence of its promoter. Gene 60(1):65-74.
2. Gerolami R. et al. 2000. Gene transfer to hepatocellular carcinoma: transduction efficacy and transgene expression kinetics by using retroviral and lentiviral vectors. Cancer Gene Ther 7(9):1286-92.
3. Lizee G. et al., 2004. Lentivirus vector-mediated expression of tumor-associated epitopes by human antigen presenting cells. Hum Gene Ther. 15(4):393-404.

All products are for internal research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Applications

in vitro or in vivo transfection

Species
Mouse
ORF size
1440 bp
Plasmid backbone
pDRIVE
Antibiotic resistance
Zeocin®
Gene promoter
PGK-1
Specificity

Ubiquitous

Subclone end
BspHI
Reporter gene
Lac Z
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Tested applications

in vitro transfection

Quality control

Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing, Plasmid DNA was purified by ion exchange chromatography

CONTENTS

Contents

  • Product: 
    pDRIVE-mPGK
  • Cat code: 
    pdrive-mpgk
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin® (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pDRIVE-mPGK

Technical Data Sheet

Plasmid Map and Sequence

Plasmid Sequence

Safety Data Sheet

Certificate of analysis

Need a CoA ?

CUSTOMER SERVICE & TECHNICAL SUPPORT

Question about this product ?