pDRIVE-EF1 / RU5'

EF1 / RU5′ promoter (Ubiquitous) in pDRIVE expression plasmid

ABOUT

EF1 / RU5′ promoter (Ubiquitous) in pDRIVE expression plasmid

Elongation Factor 1 prom / HTLV 5’UTR 

The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes.

EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

The promoter of this ’housekeeping’ gene exhibits a strong activity, higher than viral promoters such as SV40 and RSV promoters [1] and, on the contrary to the CMV promoter, yields persistent expression of the transgene in vivo [2].

InvivoGen has cloned the EF-1α promoter region of different species, among them the chimpanzee and rat.

These  sequences have not been described yet. They share respectively 98.46, 47.73 and 45.05% homology with the sequence of the human EF-1α promoter.

 

References:

1. Kim DW. et al. 1990. Use of human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene. 91(2):217-23.
2. Guo ZS. et al. 1996. Evaluation of promoter strength for hepatic gene expression in vivo following adenovirus-mediated gene transfer. Gene Ther. 3(9):802-10.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Species
Rat
Plasmid backbone
pDRIVE
Antibiotic resistance
Zeocin®
Gene promoter
EF1 / RU5′ promoter
Specificity

Ubiquitous

Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile water
Quality control

Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing.

CONTENTS

Contents

  • Product: 
    pDRIVE-rEF1/RU5'
  • Cat code: 
    pdrive-ref1ru5
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pDRIVE-rEF1/RU5'

Plasmid Map and Sequence

Safety Data Sheet

Plasmid Sequence

Technical Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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