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pDRIVE-EF1 / RU5′

pDRIVE-rEF1/RU5' Unit size Cat. code Docs Qty Price
pDRIVE bearing rat EF1 / RU5' promoter - LacZ
20 µg
pdrive-ref1ru5
+-
$495.00
pDRIVE-chEF1/RU5' Unit size Cat. code Docs Qty Price
pDRIVE bearing chimpanzee EF1 / RU5' promoter - LacZ
20 µg
pdrive-chef1ru5
+-
$495.00
pDRIVE5SEAP-rEF1/RU5' Unit size Cat. code Docs Qty Price
pDRIVE bearing rat EF1 / RU5' promoter - SEAP
20 µg
pdrive5s-ref1ru5
+-
$495.00
pDRIVE5SEAP-chEF1/RU5' Unit size Cat. code Docs Qty Price
pDRIVE bearing chimpanzee EF1 / RU5' promoter - SEAP
20 µg
pdrive5s-chef1ru5
+-
$495.00
pDRIVE5Lucia-rEF1/RU5' Unit size Cat. code Docs Qty Price
pDRIVE bearing rat EF1 / RU5' promoter - Lucia
20 µg
pdrive5lc-ref1ru5
+-
$495.00
pDRIVE5Lucia-chEF1/RU5' Unit size Cat. code Docs Qty Price
pDRIVE bearing chimpanzee EF1 / RU5' promoter - Lucia
20 µg
pdrive5lc-chef1ru5
+-
$495.00

EF1 / RU5′ promoter (Ubiquitous) in pDRIVE expression plasmid

Elongation Factor 1 prom / HTLV 5’UTR 

The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes.

EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.

The promoter of this ’housekeeping’ gene exhibits a strong activity, higher than viral promoters such as SV40 and RSV promoters [1] and, on the contrary to the CMV promoter, yields persistent expression of the transgene in vivo [2].

InvivoGen has cloned the EF-1α promoter region of different species, among them the chimpanzee and rat.

These  sequences have not been described yet. They share respectively 98.46, 47.73 and 45.05% homology with the sequence of the human EF-1α promoter.

 

References:

1. Kim DW. et al. 1990. Use of human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene. 91(2):217-23.
2. Guo ZS. et al. 1996. Evaluation of promoter strength for hepatic gene expression in vivo following adenovirus-mediated gene transfer. Gene Ther. 3(9):802-10.

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Specifications

pDRIVE bearing chimpanzee EF1 / RU5' promoter - LacZ, SEAP or Lucia

Gene Promoter: EF1 : Elongation Factor 1

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing rat EF1 / RU5' promoter - LacZ, SEAP or Lucia

Gene: EF1 : Elongation Factor 1

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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