pDRIVE-rEF1/RU5'
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Cat.code:
pdrive-ref1ru5
- Documents
ABOUT
EF1 / RU5′ promoter (Ubiquitous) in pDRIVE expression plasmid
Elongation Factor 1 prom / HTLV 5’UTR
The EF-1 alpha gene encoding elongation factor-1 alpha is an enzyme which catalyzes the GTP-dependent binding of aminoacyl-tRNA to ribosomes.
EF-1α is one of the most abundant proteins in eukaryotic cells and is expressed in almost all kinds of mammalian cells.
The promoter of this ’housekeeping’ gene exhibits a strong activity, higher than viral promoters such as SV40 and RSV promoters [1] and, on the contrary to the CMV promoter, yields persistent expression of the transgene in vivo [2].
InvivoGen has cloned the EF-1α promoter region of different species, among them the chimpanzee and rat.
These sequences have not been described yet. They share respectively 98.46, 47.73 and 45.05% homology with the sequence of the human EF-1α promoter.
References:
1. Kim DW. et al. 1990. Use of human elongation factor 1 alpha promoter as a versatile and efficient expression system. Gene. 91(2):217-23.
2. Guo ZS. et al. 1996. Evaluation of promoter strength for hepatic gene expression in vivo following adenovirus-mediated gene transfer. Gene Ther. 3(9):802-10.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Ubiquitous
Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing.
CONTENTS
Contents
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Product:pDRIVE-rEF1/RU5'
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Cat code:pdrive-ref1ru5
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Quantity:20 µg
1 ml of Zeocin™ (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Plasmid Map and Sequence
Safety Data Sheet
Plasmid Sequence
Technical Data Sheet
Certificate of analysis
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