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pDRIVE-SV40 / hFerH / mEF1

pDRIVE-SV40-hFerH-mEF1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human SV40 / hFerH / mEF1 promoter - LacZ
20 µg
pdrive-sv40ferhef1
+-
$495.00
pDRIVE5SEAP-SV40-hFerH-mEF1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human SV40 / hFerH / mEF1 promoter - SEAP
20 µg
pdrive5s-sv40ferhef1
+-
$495.00
pDRIVE5Lucia-SV40-hFerH-mEF1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human SV40 / hFerH / mEF1 promoter - Lucia
20 µg
pdrive5lc-sv40ferhef
+-
$495.00

SV40 / hFerH / mEF1 promoter (Ubiquitous) in pDRIVE expression plasmid

SV40 enh / Ferritin H prom / EF-1α 5’UTR 

Ferritin is a ubiquitous iron storage protein, whose major role is to sequester and detoxify intracellular iron that has not been utilized in cellular metabolism.

Ferritin is a 24 subunit protein composed of two subunit types, termed H (heavy) and L (light), which perform complementary functions in the protein.

The H subunit plays a role in the rapid detoxification of iron whereas the L subunit facilitates long-term iron storage. The synthesis of ferritin is highly regulated by the iron status of the cell.

The iron regulation is achieved at the translational level through interaction between a 28-nucleotide iron-responsive element (IRE), located in the 5’ untranslated region (5’UTR) of ferritin mRNAs, and a cytosolic protein, the iron regulatory protein [1].

To eliminate the iron regulation of the ferritin promoters, the 5’UTR of FerH and FerL have been replaced by the 5’UTR of the HTLV or EF-1α.

 

References:

1. Eisenstein RS & Munro HN. 1990. Translational regulation of ferritin synthesis by iron. Enzyme 44(1-4):42-58.
2. Bevilacqua MA et al. 1992. Promoter for the human ferritin heavy chain-encoding gene (FERH): structural and functional characterization. Gene 15;111(2):255-60.

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Specifications

pDRIVE bearing human SV40 / hFerH / mEF1 promoter - LacZ, SEAP or Lucia

Gene Promoter: FerH : Ferritin Heavy Chain

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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