pDRIVE-GAPDH

GAPDH promoter (Ubiquitous) in pDRIVE expression plasmid

ABOUT

GAPDH promoter (Ubiquitous) in pDRIVE expression plasmid

Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme involved in glycolysis. The GAPDH gene is constitutively expressed at high levels in almost all tissues.

However, the molecular mechanism which sustains high-level expression of this ’housekeeping’ enzyme is still unclear.

The expression of GAPDH can be induced by several physiological factors in certain cell types. In adipocytes, insulin increases the levels of GAPDH mRNA through cis-acting sequences located within the GAPDH promoter [1].

In endothelial cells, GAPDH is induced by hypoxia at the transcriptional level, this upregulation occuring to a much greater extent in EC than in other cell types [2]. 

 

References:

1. Ercolani L. et al. 1988. Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene. J Biol Chem. 263(30):15335-41.
2. Graven KK. et al. 1999. Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene. Biochim Biophys Acta. 1447(2-3):208-18.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Plasmid backbone
pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Antibiotic resistance
Zeocin®
Gene promoter
Glyceraldehyde 3-Phosphate Dehydrogenase
Specificity

Ubiquitous

Purification
Ion-exchange chromatography
Reconstitution buffer
Sterile water
Quality control

Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing.

CONTENTS

Contents

  • Product: 
    pDRIVE-hGAPDH
  • Cat code: 
    pdrive-hgapdh
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Resuspended DNA should be stored at -20°C and is stable for up to 1 year.

    Caution:

    • Avoid repeated freeze-thaw cycles

DOCUMENTS

Documents

pDRIVE-hGAPDH

Plasmid Map and Sequence

Safety Data Sheet

Plasmid Sequence

Technical Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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