pDRIVE-hGAPDH
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Cat.code:
pdrive-hgapdh
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ABOUT
GAPDH promoter (Ubiquitous) in pDRIVE expression plasmid
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme involved in glycolysis. The GAPDH gene is constitutively expressed at high levels in almost all tissues.
However, the molecular mechanism which sustains high-level expression of this ’housekeeping’ enzyme is still unclear.
The expression of GAPDH can be induced by several physiological factors in certain cell types. In adipocytes, insulin increases the levels of GAPDH mRNA through cis-acting sequences located within the GAPDH promoter [1].
In endothelial cells, GAPDH is induced by hypoxia at the transcriptional level, this upregulation occuring to a much greater extent in EC than in other cell types [2].
References:
1. Ercolani L. et al. 1988. Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene. J Biol Chem. 263(30):15335-41.
2. Graven KK. et al. 1999. Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene. Biochim Biophys Acta. 1447(2-3):208-18.
All products are for research use only, and not for human or veterinary use.
SPECIFICATIONS
Specifications
Ubiquitous
Plasmid construct has been confirmed by restriction analysis and full-length ORF sequencing.
CONTENTS
Contents
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Product:pDRIVE-hGAPDH
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Cat code:pdrive-hgapdh
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Quantity:20 µg
1 ml of Zeocin™ (100 mg/ml)
Shipping & Storage
- Shipping method: Room temperature
- -20°C
- Avoid repeated freeze-thaw cycles
Storage:
Caution:
DOCUMENTS
Documents
Plasmid Map and Sequence
Safety Data Sheet
Plasmid Sequence
Technical Data Sheet
Certificate of analysis
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