pDRIVE-GAPDH
| Product | Unit size | Cat. code | Docs. | Qty. | Price | |
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pDRIVE-hGAPDH pDRIVE bearing human GAPDH promoter - LacZ |
Show product |
20 µg |
pdrive-hgapdh
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pDRIVE5SEAP-hGAPDH pDRIVE bearing human GAPDH promoter - SEAP |
Show product |
20 µg |
pdrive5s-hgapdh
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pDRIVE5Lucia-hGAPDH pDRIVE bearing human GAPDH promoter - Lucia |
Show product |
20 µg |
pdrive5lc-hgapdh
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GAPDH promoter (Ubiquitous) in pDRIVE expression plasmid
Glyceraldehyde 3-phosphate dehydrogenase (GAPDH) is a catalytic enzyme involved in glycolysis. The GAPDH gene is constitutively expressed at high levels in almost all tissues.
However, the molecular mechanism which sustains high-level expression of this ’housekeeping’ enzyme is still unclear.
The expression of GAPDH can be induced by several physiological factors in certain cell types. In adipocytes, insulin increases the levels of GAPDH mRNA through cis-acting sequences located within the GAPDH promoter [1].
In endothelial cells, GAPDH is induced by hypoxia at the transcriptional level, this upregulation occuring to a much greater extent in EC than in other cell types [2].
References:
1. Ercolani L. et al. 1988. Isolation and complete sequence of a functional human glyceraldehyde-3-phosphate dehydrogenase gene. J Biol Chem. 263(30):15335-41.
2. Graven KK. et al. 1999. Identification of an oxygen responsive enhancer element in the glyceraldehyde-3-phosphate dehydrogenase gene. Biochim Biophys Acta. 1447(2-3):208-18.
Specifications
pDRIVE bearing human GAPDH promoter - LacZ, SEAP or Lucia
Gene Promoter: Glyceraldehyde 3-Phosphate Dehydrogenase
Specificity: Ubiquitous
Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Selection: Zeocin™
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pDRIVE contents
- 20 µg of lyophilized DNA
- 1 ml of Zeocin™ (100 mg/ml)
Product is shipped at room temperature
