pDRIVE-Ubiquitin B

Ubiquitin B promoter (Ubiquitous) in pDRIVE expression plasmid

ABOUT

Ubiquitin B promoter (Ubiquitous) in pDRIVE expression plasmid

Ubiquitin B (Ubi B) is a small highly conserved protein that is abundantly expressed in all eukaryotic cells. Ubi B is involved in the degradation of short-lived regulatory proteins as well as abnormal/mutated proteins and in the processing of major histocompatibility class I-restricted antigens [1].

A 1.1 kb fragment from the ubiquitin B gene was shown to display sustained expression of a transgene in vitro and in vivo.

Twenty days post-injection, the levels of expression were similar to those obtained with the CMV promoter and were higher 35 days post-injection [2].

 

References:

1. Ciechanover A. and Schwartz AL. 1998. The ubiquitin-proteasome pathway: the complexity and myriad functions of proteins death. Proc Natl Acad Sci USA 95(6):2727-30.
2. Yew NS. et al. 2001. High and sustained transgene expression in vivo from plasmid vectors containing a hybrid ubiquitin promoter. Mol Ther. 4(1):75-82.

All products are for research use only, and not for human or veterinary use.

SPECIFICATIONS

Specifications

Species
Human
Plasmid backbone
pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia
Antibiotic resistance
Zeocin®
Reporter gene
Lac Z
Purification
Ion-exchange chromatography
Appearance (form)
Translucent film
Appearance (color)
Colorless
Reconstitution buffer
Sterile water (not provided)
Quality control

Plasmid construct has been confirmed by restriction analysis and sequencing.

CONTENTS

Contents

  • Product: 
    pDRIVE-hUbiquitinB
  • Cat code: 
    pdrive-hubiquitinb
  • Quantity: 
    20 µg
Includes:

1 ml of Zeocin™ (100 mg/ml)

Shipping & Storage

  • Shipping method:  Room temperature
  • Storage:

    • -20°C
    Stability: Reconstituted product is stable for at least 1 year at -20 °C.

DOCUMENTS

Documents

pDRIVE-hUbiquitinB

Plasmid Map and Sequence

Safety Data Sheet

Plasmid Sequence

Technical Data Sheet

Certificate of analysis

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CUSTOMER SERVICE & TECHNICAL SUPPORT

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