pDRIVE-Ubiquitin B

Ubiquitin B promoter (Ubiquitous) in pDRIVE expression plasmid

Ubiquitin B (Ubi B) is a small highly conserved protein that is abundantly expressed in all eukaryotic cells. Ubi B is involved in the degradation of short-lived regulatory proteins as well as abnormal/mutated proteins and in the processing of major histocompatibility class I-restricted antigens [1].

A 1.1 kb fragment from the ubiquitin B gene was shown to display sustained expression of a transgene in vitro and in vivo.

Twenty days post-injection, the levels of expression were similar to those obtained with the CMV promoter and were higher 35 days post-injection [2].

References:

1. Ciechanover A. and Schwartz AL. 1998. The ubiquitin-proteasome pathway: the complexity and myriad functions of proteins death. Proc Natl Acad Sci USA 95(6):2727-30.
2. Yew NS. et al. 2001. High and sustained transgene expression in vivo from plasmid vectors containing a hybrid ubiquitin promoter. Mol Ther. 4(1):75-82.