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pDRIVE-EGR1

pDRIVE-hEGR1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human EGR1 promoter - LacZ
20 µg
pdrive-hegr1
+-
$495.00
pDRIVE5SEAP-hEGR1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human EGR1 promoter - SEAP
20 µg
pdrive5s-hegr1
+-
$495.00
pDRIVE5Lucia-hEGR1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human EGR1 promoter - Lucia
20 µg
pdrive5lc-hegr1
+-
$495.00

EGR1 promoter (Ubiquitous, radiation inducible) in pDRIVE expression plasmid

Inducible by radiation
Egr-1 is a member of the early growth response gene family like the c-fos and c-jun protooncogenes. Egr-1 expression is induced in many types of cells by various mitogenic stimuli or malignant transformation signals.

The Egr-1 promoter is known to be transiently activated by ionizing radiation. The radiation responsiveness is conferred by four CArG elements grouped in the 5’ distal region of the promoter [1].

The radiation inducibility of the Egr-1 promoter has been exploited in several experimental gene therapy strategies. In one of them, ionizing radiation was able to increase the expression of HSV1-tk 15- to 28-fold in human hepatocellular carcinoma in vitro and to significantly reduce the tumor size in nude mice [2].

 

References:

1. Datta R. et al. 1992. Ionizing radiation activates transcription of the EGR1 gene via CArG elements. Proc Natl Acad Sci USA. 89:10149-53.
2. Kawashita Y. et al. 1999. Regression of hepatocellular carcinoma in vitro and in vivo by radiosensitizing suicide gene therapy under the inducible and spatial control of radiation. Hum Gene Ther. 10(9):1509-19.

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Specifications

pDRIVE bearing human EGR1 promoter - LacZ, SEAP or Lucia

Gene Promoter: Early Growth Response 1

Specificity: Ubiquitous, radiation inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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