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pDRIVE-GRP78

pDRIVE-hGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing human GRP78 promoter - LacZ
20 µg
pdrive-hgrp78
+-
$495.00
pDRIVE5SEAP-hGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing human GRP78 promoter - SEAP
20 µg
pdrive5s-hgrp78
+-
$495.00
pDRIVE5Lucia-hGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing human GRP78 promoter - Lucia
20 µg
pdrive5lc-hgrp78
+-
$495.00
pDRIVE-mGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing mouse GRP78 promoter - LacZ
20 µg
pdrive-mgrp78
+-
$495.00
pDRIVE5SEAP-mGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing mouse GRP78 promoter - SEAP
20 µg
pdrive5s-mgrp78
+-
$495.00
pDRIVE5Lucia-mGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing mouse GRP78 promoter - Lucia
20 µg
pdrive5lc-mgrp78
+-
$495.00
pDRIVE-rGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing rat GRP78 promoter - LacZ
20 µg
pdrive-rgrp78
+-
$495.00
pDRIVE5SEAP-rGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing rat GRP78 promoter - SEAP
20 µg
pdrive5s-rgrp78
+-
$495.00
pDRIVE5Lucia-rGRP78 Unit size Cat. code Docs Qty Price
pDRIVE bearing rat GRP78 promoter - Lucia
20 µg
pdrive5lc-rgrp78
+-
$495.00

GRP78 promoter (Ubiquitous, stress-inducible) in pDRIVE expression plasmid

Stress inducible

The glucose-regulated proteins, GRP78 and GRP94, function as molecular chaperones. They are expressed constitutively in most cell types under normal growth conditions and are highly induced in stressed cells.

The genes for GRP78 and GRP94 are coordinately regulated at the transcriptional level under a variety of stress conditions. Inducing factors are cellular environments of low glucose or oxygen and reagents that disrupt the ER function such as calcium ionophores.

The GRP78 and GRP94 promoters are highly conserved and share a common regulatory domain [1]. The GRP78 promoter is known to retain its strong activity in differentiated and undifferentiated tissues whereas GRP94 is mainly effective in differentiated cells [2].

Furthermore, the GRP78 promoter can increase the expression levels of HSV-tk inside tumors resulting in complete eradication of tumor mass, with no recurrence of tumor growth [3].

 

References:

1. Chang SC. et al. 1989. Glucose-regulated protein (GRP94 and GRP78) genes share common regulatory domains and are coordinately regulated by common trans-acting factors. Mol Cell Biol 9:2153-62.
2. Kim SL. et al. 1990. Expression of the glucose-regulated proteins (GRP94 and GRP78) in differentiated and undifferentiated mouse embryonic cells and the use of the GRP78 promoter as an expression system in embryonic cells. Differentiation 42:153-9.
3. Gazit G. et al. 1999. Use of the glucose starvation-inducible glucose-regulated protein 78 promoter in suicide gene therapy of murine fibrosarcoma. Cancer Res 59: 3100-6.

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Specifications

pDRIVE bearing human GRP78 promoter - LacZ, SEAP or Lucia

Gene Promoter: Glucose-Regulated Protein 78

Specificity: Ubiquitous, stress inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing mouse GRP78 promoter - LacZ, SEAP or Lucia

Gene: Glucose-Regulated Protein 78

Specificity: Ubiquitous, stress inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing rat GRP78 promoter - LacZ, SEAP or Lucia

Gene: Glucose-Regulated Protein 78

Specificity: Ubiquitous, stress inducible

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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