pDRIVE-eIF4A1

eIF4A1 promoter (Ubiquitous) in pDRIVE expression plasmid

The eIF-4A1 gene encoding eukaryotic initiation factor 4A is ubiquitously expressed in eukaryotic cells. The minimal promoter of the eIF-4A1 gene consists of approximately 300 bp, 80% of which are identical with those in mouse eIF-4A1 promoter.

The minimal promoter contains TATA and CAAT motifs and consensus sequences binding to SP1 and AP2 [1]. The eIF-4A1 promoter is a versatile promoter giving high-levels of expression.

In some cell lines, such as macrophages, levels of expression are higher than that obtained with the CMV promoter [2].

References:

1. Kukimoto I. et al. 1997. Characterization of the cloned promoter of the human initiation factor 4AI gene. Biochem Biophys Res Commun 233(3):844-7.
2. Quinn CM. et al. 1999. The human eukaryotic initiation factor 4AI gene (EIF4A1) contains multiple regulatory elements that direct high-level reporter gene expression in mammalian cell lines. Genomics 62(3):468-76.