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pDRIVE-eIF4A1

pDRIVE-heIF4A1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human eIF4A1 promoter - LacZ
20 µg
pdrive-heif4a1
+-
$464.00
pDRIVE-meIF4A1 Unit size Cat. code Docs Qty Price
pDRIVE bearing mouse eIF4A1 promoter - LacZ
20 µg
pdrive-meif4a1
+-
$464.00
pDRIVE5SEAP-heIF4A1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human eIF4A1 promoter - SEAP
20 µg
pdrive5s-heif4a1
+-
$464.00
pDRIVE5SEAP-meIF4A1 Unit size Cat. code Docs Qty Price
pDRIVE bearing mouse eIF4A1 promoter - SEAP
20 µg
pdrive5s-meif4a1
+-
$464.00
pDRIVE5Lucia-heIF4A1 Unit size Cat. code Docs Qty Price
pDRIVE bearing human eIF4A1 promoter - Lucia
20 µg
pdrive5lc-heif4a1
+-
$464.00
pDRIVE5Lucia-meIF4A1 Unit size Cat. code Docs Qty Price
pDRIVE bearing mouse eIF4A1 promoter - Lucia
20 µg
pdrive5lc-meif4a1
+-
$464.00

eIF4A1 promoter (Ubiquitous) in pDRIVE expression plasmid

The eIF-4A1 gene encoding eukaryotic initiation factor 4A is ubiquitously expressed in eukaryotic cells. The minimal promoter of the eIF-4A1 gene consists of approximately 300 bp, 80% of which are identical with those in mouse eIF-4A1 promoter.

The minimal promoter contains TATA and CAAT motifs and consensus sequences binding to SP1 and AP2 [1]. The eIF-4A1 promoter is a versatile promoter giving high-levels of expression.

In some cell lines, such as macrophages, levels of expression are higher than that obtained with the CMV promoter [2].

 

References:

1. Kukimoto I. et al. 1997. Characterization of the cloned promoter of the human initiation factor 4AI gene. Biochem Biophys Res Commun 233(3):844-7.
2. Quinn CM. et al. 1999. The human eukaryotic initiation factor 4AI gene (EIF4A1) contains multiple regulatory elements that direct high-level reporter gene expression in mammalian cell lines. Genomics 62(3):468-76.

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Specifications

pDRIVE bearing human eIF4A1 promoter - LacZ, SEAP or Lucia

Gene Promoter: Eukaryotic Initiation Factor 4A1

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

pDRIVE bearing mouse eIF4A1 promoter - LacZ, SEAP or Lucia

Gene: Eukaryotic Initiation Factor 4A1

Specificity: Ubiquitous

Backbone: pDRIVE, pDRIVE5-SEAP or pDRIVE5-Lucia

Selection: Zeocin™

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pDRIVE contents

  • 20 µg of lyophilized DNA
  • 1 ml of Zeocin™ (100 mg/ml)

room temperature Product is shipped at room temperature

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Details

pDRIVE map

pDRIVE5-SEAP map

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