Human STING-KO Dual Reporter THP-1 Cells

STING knockout dual reporter monocytes

THP1-Dual™ KO-STING cells were generated from THP1-Dual™ cells by stable knockout of the STING gene. STING (stimulator of interferon genes), alternatively known as MPYS, TMEM173, MITA, and ERIS is a direct sensor of cyclic dinucleotides (CDNs) [1-3]. These cell lines were derived from human THP‑1 monocytes, a cell line often used to study DNA sensing pathways as they express all the cytosolic DNA sensors identified so far (with the exception of DAI).

THP1-Dual™ and THP1-Dual™ KO-STING cells stably express two inducible secreted reporter genes: IFN-inducible Lucia luciferase and NF‑κB-inducible SEAP (secreted embryonic alkaline phosphatase). They can be used to study the role of STING by monitoring IRF-induced Lucia luciferase activity, using QUANTI‑Luc™ 4 Lucia/Gaussia, a Lucia and Gaussia luciferase detection reagent.

THP1-Dual™ KO-STING cells are resistant to the selectable markers blasticidin and Zeocin®.

References:

1. Burdette DL. et al., 2011. STING is a direct innate immune sensor of cyclic di-GMP. Nature. 478(7370):515-8.
2. Zhang X. et al., 2013. Cyclic GMP-AMP containing mixed phosphodiester linkages is an endogenous high-affinity ligand for STING. Mol Cell. 51(2):226-35.
3. Barber GN. et al., 2015. STING: infection, inflammation and cancer. Nat Rev Immunol. 15(12):760-70.